Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Natasha E. Zachara; Gerald W. Hart; Robert N. Cole; Yuan Gao

doi:10.1002/0471142727.mb1706s57

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Natasha E. Zachara, Gerald W. Hart, Robert N. Cole, Yuan Gao

School of Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

Original language	English (US)
Pages (from-to)	Unit 17.6
Journal	Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Volume	Chapter 17
DOIs	https://doi.org/10.1002/0471142727.mb1706s57
State	Published - Feb 2002

ASJC Scopus subject areas

Molecular Biology

Access to Document

10.1002/0471142727.mb1706s57

Cite this

@article{084604ccf328428da246bcabd682a5b8,

title = "Detection and analysis of proteins modified by O-linked N-acetylglucosamine.",

abstract = "The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.",

author = "Zachara, {Natasha E.} and Hart, {Gerald W.} and Cole, {Robert N.} and Yuan Gao",

year = "2002",

month = feb,

doi = "10.1002/0471142727.mb1706s57",

language = "English (US)",

volume = "Chapter 17",

pages = "Unit 17.6",

journal = "Current Protocols in Molecular Biology",

issn = "1934-3639",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

AU - Zachara, Natasha E.

AU - Hart, Gerald W.

AU - Cole, Robert N.

AU - Gao, Yuan

PY - 2002/2

Y1 - 2002/2

N2 - The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

AB - The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

UR - http://www.scopus.com/inward/record.url?scp=42449115830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42449115830&partnerID=8YFLogxK

U2 - 10.1002/0471142727.mb1706s57

DO - 10.1002/0471142727.mb1706s57

M3 - Article

C2 - 18265305

AN - SCOPUS:42449115830

SN - 1934-3639

VL - Chapter 17

SP - Unit 17.6

JO - Current Protocols in Molecular Biology

JF - Current Protocols in Molecular Biology

ER -

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this