Abstract
The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.
Original language | English (US) |
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Pages (from-to) | Unit 17.6 |
Journal | Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] |
Volume | Chapter 17 |
DOIs | |
State | Published - Feb 2002 |
ASJC Scopus subject areas
- Molecular Biology