Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Natasha E. Zachara; Gerald W. Hart; Robert N. Cole; Yuan Gao

doi:10.1002/0471142727.mb1706s57

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Natasha E. Zachara, Gerald W. Hart, Robert N. Cole, Yuan Gao

School of Medicine

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10 Scopus citations

Abstract

The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

Original language	English (US)
Pages (from-to)	Unit 17.6
Journal	Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
Volume	Chapter 17
DOIs	https://doi.org/10.1002/0471142727.mb1706s57
State	Published - Feb 2002

ASJC Scopus subject areas

Molecular Biology

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abstract = "The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.",

author = "Zachara, {Natasha E.} and Hart, {Gerald W.} and Cole, {Robert N.} and Yuan Gao",

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AB - The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.

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ER -

Detection and analysis of proteins modified by O-linked N-acetylglucosamine.

Abstract

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