The modification of Ser and Thr residues with O-linked b-N-acetyl glucosamine (O-GlcNAc) is a common and essential modification of nuclear and cytoplasmic proteins, and it is thought that O-GlcNAc performs a regulatory role in the cell. This unit concentrates on the techniques for the detection and analysis of proteins modified by O-GlcNAc as well as methods for the analysis of enzymes responsible for the addition and removal of this group.
|Original language||English (US)|
|Pages (from-to)||Unit 17.6|
|Journal||Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]|
|State||Published - Feb 2002|
ASJC Scopus subject areas
- Molecular Biology