Abstract
The use of therapeutic proteins and peptides is of great interest for the treatment of many diseases, and advances in nanotechnology offer a path toward their stable delivery via preferred routes of administration. In this study, we sought to design and formulate a nanostructured lipid carrier (NLC) containing a nominal antigen (insulin peptide) for oral delivery. We utilized the design of experiments (DOE) statistical method to determine the dependencies of formulation variables on physicochemical particle characteristics including particle size, polydispersity (PDI), melting point, and latent heat of melting. The particles were determined to be non-toxic in vitro, readily taken up by primary immune cells, and found to accumulate in regional lymph nodes following oral administration. We believe that this platform technology could be broadly useful for the treatment of autoimmune diseases by supporting the development of oral delivery-based antigen specific immunotherapies.
Original language | English (US) |
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Pages (from-to) | 938-949 |
Number of pages | 12 |
Journal | Journal of Biomedical Materials Research - Part A |
Volume | 111 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2023 |
Keywords
- insulin peptide
- lipid nanoparticle
- lymphoid tissues
- oral delivery
ASJC Scopus subject areas
- Ceramics and Composites
- Metals and Alloys
- Biomedical Engineering
- Biomaterials