Demonstration of free radical generation in 'stunned' myocardium of intact dogs with the use of the spin trap α-phenyl N-tert-butyl nitrone

R. Bolli, B. S. Patel, M. O. Jeroudi, E. K. Lai, P. B. McCay

Research output: Contribution to journalArticlepeer-review

612 Scopus citations


Recent studies suggest that oxygen free radicals may mediate postischemic myocardial dysfunction ('stunning'), but all the evidence for this hypothesis is indirect. Thus, we used electron paramagnetic resonance (EPR) spectroscopy and the spin trap, α-phenyl N-tert-butyl nitrone (PBN), to directly investigate whether free radicals are produced after a 15-min coronary artery occlusion and subsequent reperfusion in 30 open-chest dogs. After intracoronary infusion of PBN, EPR signals characteristic of oxygen- and carbon-centered radical adducts were detected in the venous blood draining from the ischemic/reperfused vascular bed. The myocardial release of PBN adducts began during coronary occlusion but increased dramatically in the first few minutes after reperfusion. After this initial burst, the production of radicals abated but did not cease, persisting up to 3 h after reflow. The EPR spectra (a(β)(H) = 2.67-2.79 G, a(N) = 14.75-15.00 G) were consistent with the trapping by PBN of secondary oxygen- and carbon-centered radicals, such as alkoxy and alkyl radicals, which could be formed by reactions of primary oxygen radicals with membrane lipids. There was a linear, direct relationship between the magnitude of PBN adduct production and the degree of ischemic flow reduction. Recovery of contractile function (measured as systolic wall thickening) after reperfusion was greater (P < 0.05) in dogs given PBN than in controls. This study demonstrates that reversible regional myocardial ischemia in the intact animal is associated with prolonged free radical generation, and that the intensity of such generation is related to the severity of ischemia. The results provide direct evidence to support the hypothesis tat reactive oxygen metabolites contribute to the persistent contractile dysfunction (myocardial stunning) observed after brief ischemia in vivo.

Original languageEnglish (US)
Pages (from-to)476-485
Number of pages10
JournalJournal of Clinical Investigation
Issue number2
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Demonstration of free radical generation in 'stunned' myocardium of intact dogs with the use of the spin trap α-phenyl N-tert-butyl nitrone'. Together they form a unique fingerprint.

Cite this