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Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis

  • G. P. Melcher
  • , K. D. Reed
  • , M. G. Rinaldi
  • , J. W. Lee
  • , P. A. Pizzo
  • , T. J. Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.

Original languageEnglish (US)
Pages (from-to)192-196
Number of pages5
JournalJournal of clinical microbiology
Volume29
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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