TY - JOUR
T1 - Delivery of a viral antigen to the class I processing and presentation pathway by Listeria monocytogenes
AU - Ikonomidis, Georgios
AU - Paterson, Yvonne
AU - Kos, Ferdynand J.
AU - Portnoy, Daniel A.
PY - 1994/12/1
Y1 - 1994/12/1
N2 - Listeria monocytogenes is a facultative intracellular pathogen that grows in the cytoplasm of infected host cells. We examined the capacity of L. monocytogenes to introduce influenza nucleoprotein (NP) into the class I pathway of antigen presentation both in vitro and in vivo. Recombinant L. monocytogenes secreting a fusion of listeriolysin O and NP (LLO-NP) targeted infected cells for lysis by NP-specific class I-restricted cytotoxic T cells. Antigen presentation occurred in the context of three different class I haplotypes in vitro. A hemolysin-negative L. monocytogenes strain expressing LLO-NP was able to present in a class II-restricted manner. However, it failed to target infected cells for lysis by CD8+ T cells, indicating that hemolysin-dependent bacterial escape from the vacuole is necessary for class I presentation in vitro. Immunization of mice with a recombinant L. monocytogenes strain that stably expressed and secreted LLO-NP induced NP- specific CD8+ cytotoxic T lymphocytes. These studies have implications for the use of L. monocytogenes to deliver potentially any antigen to the class I pathway in vivo.
AB - Listeria monocytogenes is a facultative intracellular pathogen that grows in the cytoplasm of infected host cells. We examined the capacity of L. monocytogenes to introduce influenza nucleoprotein (NP) into the class I pathway of antigen presentation both in vitro and in vivo. Recombinant L. monocytogenes secreting a fusion of listeriolysin O and NP (LLO-NP) targeted infected cells for lysis by NP-specific class I-restricted cytotoxic T cells. Antigen presentation occurred in the context of three different class I haplotypes in vitro. A hemolysin-negative L. monocytogenes strain expressing LLO-NP was able to present in a class II-restricted manner. However, it failed to target infected cells for lysis by CD8+ T cells, indicating that hemolysin-dependent bacterial escape from the vacuole is necessary for class I presentation in vitro. Immunization of mice with a recombinant L. monocytogenes strain that stably expressed and secreted LLO-NP induced NP- specific CD8+ cytotoxic T lymphocytes. These studies have implications for the use of L. monocytogenes to deliver potentially any antigen to the class I pathway in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0027959410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027959410&partnerID=8YFLogxK
U2 - 10.1084/jem.180.6.2209
DO - 10.1084/jem.180.6.2209
M3 - Article
C2 - 7964496
AN - SCOPUS:0027959410
SN - 0022-1007
VL - 180
SP - 2209
EP - 2218
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 6
ER -