Deletion mapping of the regions with AMELY from two Chinese males

Jianhui Xie; Chengchen Shao; Hongmei Xu; Wei Zhu; Zhiping Liu; Qiqun Tang; Yueqin Zhou

doi:10.1016/j.legalmed.2014.05.002

Deletion mapping of the regions with AMELY from two Chinese males

Jianhui Xie, Chengchen Shao, Hongmei Xu, Wei Zhu, Zhiping Liu, Qiqun Tang, Yueqin Zhou

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The amelogenin (AMEL) is widely used in many multiplex PCR kits for gender determination. However, the null of amelogenin Y (AMELY) can result in the incorrect genotyping of male samples as females. In this study, we report the deletion of AMELY in two cases with a deletion frequency of 0.019% (2/10526) in our laboratory. The deletion region with AMELY was mapped by using other twelve loci, which shows the class I deletion pattern. Further, the Y chromosome short tandem repeat (Y-STR) typing shows that these two cases share the same haplotype with other two cases from previous reports. The haplogroup of the two cases was predicted as O3 haplogroup with a 100% probability. Altogether, this study will provide evidence to further demonstrate the deletion of AMELY in Chinese population.

Original language	English (US)
Pages (from-to)	290-292
Number of pages	3
Journal	Legal Medicine
Volume	16
Issue number	5
DOIs	https://doi.org/10.1016/j.legalmed.2014.05.002
State	Published - 2014
Externally published	Yes

Keywords

AMEL
Chinese population
Deletion
DYS458
Haplogroup

ASJC Scopus subject areas

Pathology and Forensic Medicine
Issues, ethics and legal aspects
Medicine(all)

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10.1016/j.legalmed.2014.05.002

Cite this

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title = "Deletion mapping of the regions with AMELY from two Chinese males",

abstract = "The amelogenin (AMEL) is widely used in many multiplex PCR kits for gender determination. However, the null of amelogenin Y (AMELY) can result in the incorrect genotyping of male samples as females. In this study, we report the deletion of AMELY in two cases with a deletion frequency of 0.019% (2/10526) in our laboratory. The deletion region with AMELY was mapped by using other twelve loci, which shows the class I deletion pattern. Further, the Y chromosome short tandem repeat (Y-STR) typing shows that these two cases share the same haplotype with other two cases from previous reports. The haplogroup of the two cases was predicted as O3 haplogroup with a 100% probability. Altogether, this study will provide evidence to further demonstrate the deletion of AMELY in Chinese population.",

keywords = "AMEL, Chinese population, Deletion, DYS458, Haplogroup",

author = "Jianhui Xie and Chengchen Shao and Hongmei Xu and Wei Zhu and Zhiping Liu and Qiqun Tang and Yueqin Zhou",

year = "2014",

doi = "10.1016/j.legalmed.2014.05.002",

language = "English (US)",

volume = "16",

pages = "290--292",

journal = "Legal Medicine",

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number = "5",

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TY - JOUR

T1 - Deletion mapping of the regions with AMELY from two Chinese males

AU - Xie, Jianhui

AU - Shao, Chengchen

AU - Xu, Hongmei

AU - Zhu, Wei

AU - Liu, Zhiping

AU - Tang, Qiqun

AU - Zhou, Yueqin

PY - 2014

Y1 - 2014

N2 - The amelogenin (AMEL) is widely used in many multiplex PCR kits for gender determination. However, the null of amelogenin Y (AMELY) can result in the incorrect genotyping of male samples as females. In this study, we report the deletion of AMELY in two cases with a deletion frequency of 0.019% (2/10526) in our laboratory. The deletion region with AMELY was mapped by using other twelve loci, which shows the class I deletion pattern. Further, the Y chromosome short tandem repeat (Y-STR) typing shows that these two cases share the same haplotype with other two cases from previous reports. The haplogroup of the two cases was predicted as O3 haplogroup with a 100% probability. Altogether, this study will provide evidence to further demonstrate the deletion of AMELY in Chinese population.

AB - The amelogenin (AMEL) is widely used in many multiplex PCR kits for gender determination. However, the null of amelogenin Y (AMELY) can result in the incorrect genotyping of male samples as females. In this study, we report the deletion of AMELY in two cases with a deletion frequency of 0.019% (2/10526) in our laboratory. The deletion region with AMELY was mapped by using other twelve loci, which shows the class I deletion pattern. Further, the Y chromosome short tandem repeat (Y-STR) typing shows that these two cases share the same haplotype with other two cases from previous reports. The haplogroup of the two cases was predicted as O3 haplogroup with a 100% probability. Altogether, this study will provide evidence to further demonstrate the deletion of AMELY in Chinese population.

KW - AMEL

KW - Chinese population

KW - Deletion

KW - DYS458

KW - Haplogroup

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JO - Legal Medicine

JF - Legal Medicine

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Deletion mapping of the regions with AMELY from two Chinese males

Abstract

Keywords

ASJC Scopus subject areas

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