TY - JOUR
T1 - Definition of the range and distribution of human immunodeficiency virus macrophage tropism using PCR-based infectivity measurements
AU - Collin, M.
AU - Illei, P.
AU - James, W.
AU - Gordon, S.
PY - 1994
Y1 - 1994
N2 - The tropism of human immunodeficiency virus (HIV) for macrophages (m∅) is a well recognized phenomenon, but the range and distribution of m∅-tropic phenotypes have not been defined by quantitative means. This study uses a PCR-based infectivity assay to derive an index of m∅ tropism for several common strains of HIV. The results show that m∅ tropism varies over about six orders of magnitude and that the most m∅-tropic strains have a higher infectivity for m∅ than for peripheral blood lymphocytes. Strains were distributed throughout this range, suggesting that m∅ tropism is a continuously variable phenotypic property. Although the degree of tropism was strongly influenced by the mode of isolation and propagation of virus strains, there was no evidence for the existence of distinct m∅-tropic or non-m∅-tropic phenotypes. Finally, the tropism of two selected strains was found to be determined by an early step in replication, probably virus entry.
AB - The tropism of human immunodeficiency virus (HIV) for macrophages (m∅) is a well recognized phenomenon, but the range and distribution of m∅-tropic phenotypes have not been defined by quantitative means. This study uses a PCR-based infectivity assay to derive an index of m∅ tropism for several common strains of HIV. The results show that m∅ tropism varies over about six orders of magnitude and that the most m∅-tropic strains have a higher infectivity for m∅ than for peripheral blood lymphocytes. Strains were distributed throughout this range, suggesting that m∅ tropism is a continuously variable phenotypic property. Although the degree of tropism was strongly influenced by the mode of isolation and propagation of virus strains, there was no evidence for the existence of distinct m∅-tropic or non-m∅-tropic phenotypes. Finally, the tropism of two selected strains was found to be determined by an early step in replication, probably virus entry.
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U2 - 10.1099/0022-1317-75-7-1597
DO - 10.1099/0022-1317-75-7-1597
M3 - Article
C2 - 8021591
AN - SCOPUS:0028217679
SN - 0022-1317
VL - 75
SP - 1597
EP - 1603
JO - Journal of General Virology
JF - Journal of General Virology
IS - 7
ER -