Abstract
We have used an Escherichia coli K-12 whole-genome array based on the DNA sequence of strain MG1655 as a tool to identify deletions in another E. coli K-12 strain, MC4100, by probing the array with labeled chromosomal DNA. Despite the continued widespread use of MC4100 as an experimental system, the specific genetic relationship of this strain to the sequenced K-12 derivative MG1655 has not been resolved. MC4100 was found to contain four deletions, ranging from 1 to 97 kb in size. The exact nature of three of the deletions was previously unresolved, and the fourth deletion was altogether unknown.
Original language | English (US) |
---|---|
Pages (from-to) | 2017-2021 |
Number of pages | 5 |
Journal | Journal of bacteriology |
Volume | 185 |
Issue number | 6 |
DOIs | |
State | Published - Mar 2003 |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology