Defining system requirements for simplified blood culture to enable widespread use in resource-limited settings

Peter J. Dailey, Jennifer Osborn, Elizabeth A. Ashley, Ellen Jo Baron, David A.B. Dance, Daniela Fusco, Caterina Fanello, Yukari C. Manabe, Margaret Mokomane, Paul N. Newton, Belay Tessema, Chris Isaacs, Sabine Dittrich

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Bacterial blood stream infections (BSI) are a common cause of mortality and morbidity globally. As the causative agents and the resulting treatment decisions vary, near-patient testing and surveillance tools are necessary to monitor bacterial causes and resistance to antimicrobial agents. The gold standard to identify BSIs is blood culture (BC), a methodology not widely available in resource-limited settings. The aim of the study was to map out a target product profile of a simplified BC system (SBCS) to inform product development efforts. To identify the desired characteristics of a SBCS, we enlisted a small group of specialists working in Africa and Asia. Questions were used to understand challenges and how these constraints inform system requirements. The specialists were infectious disease physicians, public health/clinical microbiologists, clinical researchers, and technology experts with different geographical backgrounds. All suggested that BC should ideally be available at the district hospital level. Many of the same operational challenges, such as limited availability of culture bottles, electricity and internet connectivity, profuse dust, the lack of ambient temperature control, and human capacity constraints were identified across the different regions. BCs, although the accepted gold standard for diagnosis of BSIs, are not widely available outside of reference/research centers in Africa and Asia. To extend the reach of this important tool, it is crucial to engage product developers and academic research partners to develop accessible alternatives.

Original languageEnglish (US)
Article number10
Issue number1
StatePublished - Mar 1 2019


  • AMR
  • Blood culture
  • Hemoculture
  • LMICs
  • Low- and middle-income countries
  • Microbiology
  • Resource limited
  • Sepsis
  • Target product profile

ASJC Scopus subject areas

  • Clinical Biochemistry


Dive into the research topics of 'Defining system requirements for simplified blood culture to enable widespread use in resource-limited settings'. Together they form a unique fingerprint.

Cite this