DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA

Jun Gao; Alicia K. Byrd; Boris L. Zybailov; John C. Marecki; Michael J. Guderyon; Andrea D. Edwards; Shubeena Chib; Kirk L. West; Zachary J. Waldrip; Samuel G. Mackintosh; Zhaofeng Gao; Andrea A. Putnam; Eckhard Jankowsky; Kevin D. Raney

doi:10.1039/c8cc10091h

DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA

Jun Gao
, Alicia K. Byrd
, Boris L. Zybailov
, John C. Marecki
, Michael J. Guderyon
, Andrea D. Edwards
, Shubeena Chib
, Kirk L. West
, Zachary J. Waldrip
, Samuel G. Mackintosh
, Zhaofeng Gao
, Andrea A. Putnam
, Eckhard Jankowsky
, Kevin D. Raney

Research output: Contribution to journal › Article › peer-review

Abstract

We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.

Original language	English (US)
Pages (from-to)	4467-4470
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	31
DOIs	https://doi.org/10.1039/c8cc10091h
State	Published - 2019
Externally published	Yes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c8cc10091h

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Gao, J., Byrd, A. K., Zybailov, B. L., Marecki, J. C., Guderyon, M. J., Edwards, A. D., Chib, S., West, K. L., Waldrip, Z. J., Mackintosh, S. G., Gao, Z., Putnam, A. A., Jankowsky, E., & Raney, K. D. (2019). DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA. Chemical Communications, 55(31), 4467-4470. https://doi.org/10.1039/c8cc10091h

Gao, J, Byrd, AK, Zybailov, BL, Marecki, JC, Guderyon, MJ, Edwards, AD, Chib, S, West, KL, Waldrip, ZJ, Mackintosh, SG, Gao, Z, Putnam, AA, Jankowsky, E & Raney, KD 2019, 'DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA', Chemical Communications, vol. 55, no. 31, pp. 4467-4470. https://doi.org/10.1039/c8cc10091h

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title = "DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA",

abstract = "We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.",

author = "Jun Gao and Byrd, \{Alicia K.\} and Zybailov, \{Boris L.\} and Marecki, \{John C.\} and Guderyon, \{Michael J.\} and Edwards, \{Andrea D.\} and Shubeena Chib and West, \{Kirk L.\} and Waldrip, \{Zachary J.\} and Mackintosh, \{Samuel G.\} and Zhaofeng Gao and Putnam, \{Andrea A.\} and Eckhard Jankowsky and Raney, \{Kevin D.\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

year = "2019",

doi = "10.1039/c8cc10091h",

language = "English (US)",

volume = "55",

pages = "4467--4470",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA

AU - Gao, Jun

AU - Byrd, Alicia K.

AU - Zybailov, Boris L.

AU - Marecki, John C.

AU - Guderyon, Michael J.

AU - Edwards, Andrea D.

AU - Chib, Shubeena

AU - West, Kirk L.

AU - Waldrip, Zachary J.

AU - Mackintosh, Samuel G.

AU - Gao, Zhaofeng

AU - Putnam, Andrea A.

AU - Jankowsky, Eckhard

AU - Raney, Kevin D.

PY - 2019

Y1 - 2019

N2 - We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.

AB - We identified 29 G-quadruplex binding proteins by affinity purification and quantitative LC-MS/MS. We demonstrated that the DEAD-box RNA helicases Dbp2, Ded1 and Mss116 preferentially bind to G-quadruplex nucleic acids in vitro and destabilize RNA quadruplexes, suggesting new potential roles for these helicases in disruption of quadruplex structures in RNA.

UR - https://www.scopus.com/pages/publications/85064191477

UR - https://www.scopus.com/pages/publications/85064191477#tab=citedBy

U2 - 10.1039/c8cc10091h

DO - 10.1039/c8cc10091h

M3 - Article

C2 - 30855040

AN - SCOPUS:85064191477

SN - 1359-7345

VL - 55

SP - 4467

EP - 4470

JO - Chemical Communications

JF - Chemical Communications

IS - 31

ER -

DEAD-box RNA helicases Dbp2, Ded1 and Mss116 bind to G-quadruplex nucleic acids and destabilize G-quadruplex RNA

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