D-Titin: A giant protein with dual roles in chromosomes and muscles

Cristina Machado, Deborah J. Andrew

Research output: Contribution to journalArticlepeer-review

114 Scopus citations


Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH2-terminal half of vertebrate titin. Mutations in D-Titin cause chromosome undercondensation, chromosome breakage, loss of diploidy, and premature sister chromatid separation. Additionally, D-Titin mutants have defects in myoblast fusion and muscle organization. The phenotypes of the D-Titin mutants suggest parallel roles for titin in both muscle and chromosome structure and elasticity, and provide new insight into chromosome structure.

Original languageEnglish (US)
Pages (from-to)639-651
Number of pages13
JournalJournal of Cell Biology
Issue number3
StatePublished - Oct 30 2000


  • Chromosome condensation
  • Drosophila melanogaster
  • Myoblast fusion
  • Sarcomere
  • Titin

ASJC Scopus subject areas

  • Cell Biology


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