Cytotoxic effects of anticancer agents on subconfluent and multilayered postconfluent cultures

Paulo E. Pizao, Godefridus J. Peters, Jannette Van Ark-Otte, Lou A. Smets, Evelien Smitskamp-Wilms, Benjamin Winograd, Herbert M. Pinedo, Giuseppe Giaccone

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


The cytotoxic effects of conventional (doxorubicin, 5-fluorouracil, cisplatin) and investigational (2′,2′-difluorodeoxycytidine, hexadecylphosphocholine, EO9, rhizoxin) anticancer drugs were studied in subconfluent and multilayered postconfluent cultures of human colon and ovarian carcinoma cell lines. Chemosensitivity was assessed 4 days after a 24-h drug exposure with the sulphorhodamine B assay. Except for rhizoxin, all drugs tested yielded an ec50 (drug concentration producing absorbance readings 50% lower than those of non-treated wells) in postconfluent cultures that were higher than an ec50 obtained with subconfluent cultures. Compared with subconfluent cultures, postconfluent cultures showed decreased cellular nucleotide concentrations and ATP/ADP ratios, in addition to an increased percentage of G0/G1 activity of DT-diaphorase, a reductase involved in the bioactivation of EO9, was similar in sub- and postconfluent cultures. These results indicate similarity of the postconfluent model presented with those obtained with in vivo models and more complex in vitro techniques.

Original languageEnglish (US)
Pages (from-to)1566-1573
Number of pages8
JournalEuropean Journal of Cancer
Issue number11
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology


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