Cytoplasmic Actin Is an Extracellular Insect Immune Factor which Is Secreted upon Immune Challenge and Mediates Phagocytosis and Direct Killing of Bacteria, and Is a Plasmodium Antagonist

Simone L. Sandiford; Yuemei Dong; Andrew Pike; Benjamin J. Blumberg; Ana C. Bahia; George Dimopoulos

doi:10.1371/journal.ppat.1004631

Cytoplasmic Actin Is an Extracellular Insect Immune Factor which Is Secreted upon Immune Challenge and Mediates Phagocytosis and Direct Killing of Bacteria, and Is a Plasmodium Antagonist

Simone L. Sandiford, Yuemei Dong, Andrew Pike, Benjamin J. Blumberg, Ana C. Bahia, George Dimopoulos

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Actin is a highly versatile, abundant, and conserved protein, with functions in a variety of intracellular processes. Here, we describe a novel role for insect cytoplasmic actin as an extracellular pathogen recognition factor that mediates antibacterial defense. Insect actins are secreted from cells upon immune challenge through an exosome-independent pathway. Anopheles gambiae actin interacts with the extracellular MD2-like immune factor AgMDL1, and binds to the surfaces of bacteria, mediating their phagocytosis and direct killing. Globular and filamentous actins display distinct functions as extracellular immune factors, and mosquito actin is a Plasmodium infection antagonist.

Original language	English (US)
Article number	e1004631
Journal	PLoS pathogens
Volume	11
Issue number	2
DOIs	https://doi.org/10.1371/journal.ppat.1004631
State	Published - 2015

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Molecular Biology
Genetics
Virology

Access to Document

10.1371/journal.ppat.1004631

Cite this

@article{5f00b181028c41ae8b7ee521eb7d6388,

title = "Cytoplasmic Actin Is an Extracellular Insect Immune Factor which Is Secreted upon Immune Challenge and Mediates Phagocytosis and Direct Killing of Bacteria, and Is a Plasmodium Antagonist",

abstract = "Actin is a highly versatile, abundant, and conserved protein, with functions in a variety of intracellular processes. Here, we describe a novel role for insect cytoplasmic actin as an extracellular pathogen recognition factor that mediates antibacterial defense. Insect actins are secreted from cells upon immune challenge through an exosome-independent pathway. Anopheles gambiae actin interacts with the extracellular MD2-like immune factor AgMDL1, and binds to the surfaces of bacteria, mediating their phagocytosis and direct killing. Globular and filamentous actins display distinct functions as extracellular immune factors, and mosquito actin is a Plasmodium infection antagonist.",

author = "Sandiford, {Simone L.} and Yuemei Dong and Andrew Pike and Blumberg, {Benjamin J.} and Bahia, {Ana C.} and George Dimopoulos",

note = "Publisher Copyright: {\textcopyright} 2015 Sandiford et al.",

year = "2015",

doi = "10.1371/journal.ppat.1004631",

language = "English (US)",

volume = "11",

journal = "PLoS pathogens",

issn = "1553-7366",

publisher = "Public Library of Science",

number = "2",

}

TY - JOUR

T1 - Cytoplasmic Actin Is an Extracellular Insect Immune Factor which Is Secreted upon Immune Challenge and Mediates Phagocytosis and Direct Killing of Bacteria, and Is a Plasmodium Antagonist

AU - Sandiford, Simone L.

AU - Dong, Yuemei

AU - Pike, Andrew

AU - Blumberg, Benjamin J.

AU - Bahia, Ana C.

AU - Dimopoulos, George

PY - 2015

Y1 - 2015

N2 - Actin is a highly versatile, abundant, and conserved protein, with functions in a variety of intracellular processes. Here, we describe a novel role for insect cytoplasmic actin as an extracellular pathogen recognition factor that mediates antibacterial defense. Insect actins are secreted from cells upon immune challenge through an exosome-independent pathway. Anopheles gambiae actin interacts with the extracellular MD2-like immune factor AgMDL1, and binds to the surfaces of bacteria, mediating their phagocytosis and direct killing. Globular and filamentous actins display distinct functions as extracellular immune factors, and mosquito actin is a Plasmodium infection antagonist.

AB - Actin is a highly versatile, abundant, and conserved protein, with functions in a variety of intracellular processes. Here, we describe a novel role for insect cytoplasmic actin as an extracellular pathogen recognition factor that mediates antibacterial defense. Insect actins are secreted from cells upon immune challenge through an exosome-independent pathway. Anopheles gambiae actin interacts with the extracellular MD2-like immune factor AgMDL1, and binds to the surfaces of bacteria, mediating their phagocytosis and direct killing. Globular and filamentous actins display distinct functions as extracellular immune factors, and mosquito actin is a Plasmodium infection antagonist.

UR - http://www.scopus.com/inward/record.url?scp=84924368514&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924368514&partnerID=8YFLogxK

U2 - 10.1371/journal.ppat.1004631

DO - 10.1371/journal.ppat.1004631

M3 - Article

C2 - 25658622

AN - SCOPUS:84924368514

SN - 1553-7366

VL - 11

JO - PLoS pathogens

JF - PLoS pathogens

IS - 2

M1 - e1004631

ER -

Cytoplasmic Actin Is an Extracellular Insect Immune Factor which Is Secreted upon Immune Challenge and Mediates Phagocytosis and Direct Killing of Bacteria, and Is a Plasmodium Antagonist

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this