Cytomorphometry. A methodologic study of preparation techniques, selection methods and sample sizes

P. J. Van Diest, A. W M Smeulders, J. P A Baak

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The influence of methodologic aspects on cytomorphometric features was studied using preparations of hepatoma and/or mastocytoma cells. First, two preparation techniques (smear and oese ) were compared. Second, four methods of selecting cells for cytomorphometric analysis (two conventional and two stratified methods) were tested for reproducibility. Third, heterogeneous cell populations were used to estimate the required sample size using the running coefficient of variation(CV), and the resutls were compared with expected ( theoretical) values of the required samle size calculated using the standard error of the mean. The results showed significantly lower CVs for the smear preparation technique. The stratified methods appeared to be superior to the conventional methods for selecting cells for measurement. The experimentally assessed sample sizes were considerably lower than the corresponding theoretical calculations. These findings suggest that morphometric assessments in cytologic smears should utilize a stratified cell selection method. While experimentally assessed sample sizes are relatively small and therefore better routinely applicable, they may yield less reliable results in some cases. The need to test a sample for its reproducibility as well as its discriminatory power is emphasized.

Original languageEnglish (US)
Pages (from-to)225-231
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Issue number4
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Cell Biology


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