Cytokine and chemokine profiles following vaccination with human papillomavirus type 16 L1 virus-like particles

Alfonso García-Piñeres, Allan Hildesheim, Lori Dodd, Troy J. Kemp, Marcus Williams, Clayton Harro, Douglas R. Lowy, John T. Schiller, Ligia A. Pinto

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


To determine the systemic cytokine pattern induced by vaccination with human papillomavirus (HPV) L1 virus-like particles (VLP), we analyzed 22 different cytokines in culture supernatants of L1 VLP-stimulated peripheral blood mononuclear cells from vaccine (n = 19) and placebo (n = 7) recipients at months 0 and 2 after vaccination, using a multiplex cytokine bead array. In vaccine recipients, incubation with L1 VLP in vitro led to a statistically significant increase in production of Th1 (granulocyte-macrophage colony-stimulating factor, interleukin-2 [IL-2], gamma interferon; P < 0.0007) and Th2 (IL-4, IL-5, IL-10, IL-13; P < 0.0017) cytokines and the chemokine IP-10 (P = 0.0021) at month 2 after immunization, compared to levels seen prior to vaccination. These responses were not seen in placebo recipients. Cytokine and neutralizing antibody responses to vaccination followed the same pattern, with the highest antibody responses seen for subjects with higher cytokine responses. Cytokine profiling studies using samples from efficacy trials may provide important information about discriminators of long-term protection against HPV.

Original languageEnglish (US)
Pages (from-to)984-989
Number of pages6
JournalClinical and Vaccine Immunology
Issue number8
StatePublished - Aug 2007
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)


Dive into the research topics of 'Cytokine and chemokine profiles following vaccination with human papillomavirus type 16 L1 virus-like particles'. Together they form a unique fingerprint.

Cite this