TY - JOUR
T1 - Cytokeratin expression in corneal dystrophies
AU - Coutinho, Anamaria Baptista
AU - De Freitas, Denise
AU - Filho, João Pessoa De Souza
AU - Corrêa, Zélia Maria S.
AU - Odashiro, Alexandre Nakao
AU - Burnier, Miguel N.
PY - 2011/3
Y1 - 2011/3
N2 - Purpose: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. Methods: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on paraffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). Results: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. Conclusion: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.
AB - Purpose: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. Methods: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on paraffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). Results: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. Conclusion: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.
KW - Cornea/pathology
KW - Corneal dystrophies
KW - Hereditary
KW - Immunohistochemistry
KW - Keratins/physiology
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U2 - 10.1590/S0004-27492011000200010
DO - 10.1590/S0004-27492011000200010
M3 - Article
C2 - 21779668
AN - SCOPUS:79961123501
SN - 0004-2749
VL - 74
SP - 118
EP - 122
JO - Arquivos brasileiros de oftalmologia
JF - Arquivos brasileiros de oftalmologia
IS - 2
ER -