CyTOF protocol for immune monitoring of solid tumors from mouse models

Sarah M. Shin; Alexei Hernandez; Erin Coyne; Kabeer Munjal; Nicole E. Gross; Soren Charmsaz; Xuan Yuan; Hongqui Yang; Won Jin Ho

doi:10.1016/j.xpro.2022.101949

CyTOF protocol for immune monitoring of solid tumors from mouse models

Sarah M. Shin, Alexei Hernandez, Erin Coyne, Kabeer Munjal, Nicole E. Gross, Soren Charmsaz, Xuan Yuan, Hongqui Yang, Won Jin Ho

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects. For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).¹

Original language	English (US)
Article number	101949
Journal	STAR Protocols
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2022.101949
State	Published - Mar 17 2023

Keywords

Antibody
Cancer
Flow Cytometry/Mass Cytometry
Immunology

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology
General Neuroscience

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10.1016/j.xpro.2022.101949

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title = "CyTOF protocol for immune monitoring of solid tumors from mouse models",

abstract = "Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects. For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).1",

keywords = "Antibody, Cancer, Flow Cytometry/Mass Cytometry, Immunology",

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AU - Shin, Sarah M.

AU - Hernandez, Alexei

AU - Coyne, Erin

AU - Munjal, Kabeer

AU - Gross, Nicole E.

AU - Charmsaz, Soren

AU - Yuan, Xuan

AU - Yang, Hongqui

AU - Ho, Won Jin

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects. For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).1

AB - Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects. For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).1

KW - Antibody

KW - Cancer

KW - Flow Cytometry/Mass Cytometry

KW - Immunology

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CyTOF protocol for immune monitoring of solid tumors from mouse models

Abstract

Keywords

ASJC Scopus subject areas

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