Cysteinyl-tRNA formation: The last puzzle of aminoacyl-tRNA synthesis

Tong Li; David E. Graham; Constantinos Stathopoulos; Paul J. Haney; Hyun Soo Kim; Ute Vothknecht; Makoto Kitabatake; Kwang Won Hong; Gudmundur Eggertsson; Alan W. Curnow; Winston Lin; Ivana Celic; William Whitman; Dieter Söll

doi:10.1016/S0014-5793(99)01550-1

Cysteinyl-tRNA formation: The last puzzle of aminoacyl-tRNA synthesis

Tong Li, David E. Graham, Constantinos Stathopoulos, Paul J. Haney, Hyun Soo Kim, Ute Vothknecht, Makoto Kitabatake, Kwang Won Hong, Gudmundur Eggertsson, Alan W. Curnow, Winston Lin, Ivana Celic, William Whitman, Dieter Söll

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

With the exception of the methanogenic archaea Methanococcus jannaschii and Methanobacterium thermoautotrophicum ΔH, all organisms surveyed contain orthologs of Escherichia coli cysteinyl-tRNA synthetase (CysRS). The characterization of CysRS-encoding (cysS) genes and the demonstration of their ability to complement an E. coli cysS(ts) mutant reveal that Methanococcus maripaludis and Methanosarcina barkeri, two other methanogenic archaea, possess canonical CysRS proteins. A molecular phylogeny inferred from 40 CysRS sequences indicates that the CysRS of M. maripaludis and Methanosarcina spp. are specific relatives of the CysRS of Pyrococcus spp. and Chlamydia, respectively. This result suggests that the CysRS gene was acquired by lateral gene transfer in at least one euryarchaeotic lineage. Copyright (C) 1999 Federation of European Biochemical Societies.

Original language	English (US)
Pages (from-to)	302-306
Number of pages	5
Journal	FEBS Letters
Volume	462
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01550-1
State	Published - Dec 3 1999
Externally published	Yes

Keywords

Aminoacyl-tRNA
Cysteinyl-tRNA synthetase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)01550-1

Cite this

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title = "Cysteinyl-tRNA formation: The last puzzle of aminoacyl-tRNA synthesis",

abstract = "With the exception of the methanogenic archaea Methanococcus jannaschii and Methanobacterium thermoautotrophicum ΔH, all organisms surveyed contain orthologs of Escherichia coli cysteinyl-tRNA synthetase (CysRS). The characterization of CysRS-encoding (cysS) genes and the demonstration of their ability to complement an E. coli cysS(ts) mutant reveal that Methanococcus maripaludis and Methanosarcina barkeri, two other methanogenic archaea, possess canonical CysRS proteins. A molecular phylogeny inferred from 40 CysRS sequences indicates that the CysRS of M. maripaludis and Methanosarcina spp. are specific relatives of the CysRS of Pyrococcus spp. and Chlamydia, respectively. This result suggests that the CysRS gene was acquired by lateral gene transfer in at least one euryarchaeotic lineage. Copyright (C) 1999 Federation of European Biochemical Societies.",

keywords = "Aminoacyl-tRNA, Cysteinyl-tRNA synthetase",

author = "Tong Li and Graham, {David E.} and Constantinos Stathopoulos and Haney, {Paul J.} and Kim, {Hyun Soo} and Ute Vothknecht and Makoto Kitabatake and Hong, {Kwang Won} and Gudmundur Eggertsson and Curnow, {Alan W.} and Winston Lin and Ivana Celic and William Whitman and Dieter S{\"o}ll",

note = "Funding Information: We thank S. Fitz-Gibbon, T. Hartsch, D. Oesterhelt, A. Ruepp and S. Schuster for sharing unpublished sequence data. M.K. was a postdoctoral fellow of the Japan Society for the Promotion of Science. D.E.G. was supported by an NIH Cellular and Molecular Biology Training grant. This work was supported by grants from the National Institute of General Medical Sciences (to D.S.) and the U.S. Department of Energy (to D.S. and W.B.W.). ",

year = "1999",

month = dec,

day = "3",

doi = "10.1016/S0014-5793(99)01550-1",

language = "English (US)",

volume = "462",

pages = "302--306",

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number = "3",

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TY - JOUR

T1 - Cysteinyl-tRNA formation

T2 - The last puzzle of aminoacyl-tRNA synthesis

AU - Li, Tong

AU - Graham, David E.

AU - Stathopoulos, Constantinos

AU - Haney, Paul J.

AU - Kim, Hyun Soo

AU - Vothknecht, Ute

AU - Kitabatake, Makoto

AU - Hong, Kwang Won

AU - Eggertsson, Gudmundur

AU - Curnow, Alan W.

AU - Lin, Winston

AU - Celic, Ivana

AU - Whitman, William

AU - Söll, Dieter

N1 - Funding Information: We thank S. Fitz-Gibbon, T. Hartsch, D. Oesterhelt, A. Ruepp and S. Schuster for sharing unpublished sequence data. M.K. was a postdoctoral fellow of the Japan Society for the Promotion of Science. D.E.G. was supported by an NIH Cellular and Molecular Biology Training grant. This work was supported by grants from the National Institute of General Medical Sciences (to D.S.) and the U.S. Department of Energy (to D.S. and W.B.W.).

PY - 1999/12/3

Y1 - 1999/12/3

N2 - With the exception of the methanogenic archaea Methanococcus jannaschii and Methanobacterium thermoautotrophicum ΔH, all organisms surveyed contain orthologs of Escherichia coli cysteinyl-tRNA synthetase (CysRS). The characterization of CysRS-encoding (cysS) genes and the demonstration of their ability to complement an E. coli cysS(ts) mutant reveal that Methanococcus maripaludis and Methanosarcina barkeri, two other methanogenic archaea, possess canonical CysRS proteins. A molecular phylogeny inferred from 40 CysRS sequences indicates that the CysRS of M. maripaludis and Methanosarcina spp. are specific relatives of the CysRS of Pyrococcus spp. and Chlamydia, respectively. This result suggests that the CysRS gene was acquired by lateral gene transfer in at least one euryarchaeotic lineage. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - With the exception of the methanogenic archaea Methanococcus jannaschii and Methanobacterium thermoautotrophicum ΔH, all organisms surveyed contain orthologs of Escherichia coli cysteinyl-tRNA synthetase (CysRS). The characterization of CysRS-encoding (cysS) genes and the demonstration of their ability to complement an E. coli cysS(ts) mutant reveal that Methanococcus maripaludis and Methanosarcina barkeri, two other methanogenic archaea, possess canonical CysRS proteins. A molecular phylogeny inferred from 40 CysRS sequences indicates that the CysRS of M. maripaludis and Methanosarcina spp. are specific relatives of the CysRS of Pyrococcus spp. and Chlamydia, respectively. This result suggests that the CysRS gene was acquired by lateral gene transfer in at least one euryarchaeotic lineage. Copyright (C) 1999 Federation of European Biochemical Societies.

KW - Aminoacyl-tRNA

KW - Cysteinyl-tRNA synthetase

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Cysteinyl-tRNA formation: The last puzzle of aminoacyl-tRNA synthesis

Abstract

Keywords

ASJC Scopus subject areas

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