Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells

H. J. Broxterman; H. M. Pinedo; G. J. Schuurhuis; J. Lankelma

Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells

H. J. Broxterman, H. M. Pinedo, G. J. Schuurhuis, J. Lankelma

Research output: Contribution to journal › Article › peer-review

Abstract

Cyclosporin A (Sandimmune®) rapidly induced an increase in daunorubicin accumulation in multidrug-resistant human ovarian carcinoma cells (2780AD) and was more potent than verapamil. Steady-state ³H-cyclosporin A accumulation at 37°C in 2780AD cells was 60-70% of that in the sensitive A2780 cells. A rapid increase of ATP consumption and lactate production was induced in 2780AD cells by verapamil, but not by cyclosporin A. These results suggest that the interactions of cyclosporin A and verapamil with P-glycoprotein, which leads to inhibition of drug transport, have a different mechanistic basis.

Original language	English (US)
Pages (from-to)	85-88
Number of pages	4
Journal	British Journal of Cancer
Volume	62
Issue number	1
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

Cancer Research
Oncology

Cite this

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title = "Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells",

abstract = "Cyclosporin A (Sandimmune{\textregistered}) rapidly induced an increase in daunorubicin accumulation in multidrug-resistant human ovarian carcinoma cells (2780AD) and was more potent than verapamil. Steady-state 3H-cyclosporin A accumulation at 37°C in 2780AD cells was 60-70% of that in the sensitive A2780 cells. A rapid increase of ATP consumption and lactate production was induced in 2780AD cells by verapamil, but not by cyclosporin A. These results suggest that the interactions of cyclosporin A and verapamil with P-glycoprotein, which leads to inhibition of drug transport, have a different mechanistic basis.",

author = "Broxterman, {H. J.} and Pinedo, {H. M.} and Schuurhuis, {G. J.} and J. Lankelma",

year = "1990",

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T1 - Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells

AU - Broxterman, H. J.

AU - Pinedo, H. M.

AU - Schuurhuis, G. J.

AU - Lankelma, J.

PY - 1990

Y1 - 1990

N2 - Cyclosporin A (Sandimmune®) rapidly induced an increase in daunorubicin accumulation in multidrug-resistant human ovarian carcinoma cells (2780AD) and was more potent than verapamil. Steady-state 3H-cyclosporin A accumulation at 37°C in 2780AD cells was 60-70% of that in the sensitive A2780 cells. A rapid increase of ATP consumption and lactate production was induced in 2780AD cells by verapamil, but not by cyclosporin A. These results suggest that the interactions of cyclosporin A and verapamil with P-glycoprotein, which leads to inhibition of drug transport, have a different mechanistic basis.

AB - Cyclosporin A (Sandimmune®) rapidly induced an increase in daunorubicin accumulation in multidrug-resistant human ovarian carcinoma cells (2780AD) and was more potent than verapamil. Steady-state 3H-cyclosporin A accumulation at 37°C in 2780AD cells was 60-70% of that in the sensitive A2780 cells. A rapid increase of ATP consumption and lactate production was induced in 2780AD cells by verapamil, but not by cyclosporin A. These results suggest that the interactions of cyclosporin A and verapamil with P-glycoprotein, which leads to inhibition of drug transport, have a different mechanistic basis.

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JF - British Journal of Cancer

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ER -

Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells

Abstract

ASJC Scopus subject areas

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