Cyclin D1-Cdk4 controls glucose metabolism independently of cell cycle progression

Yoonjin Lee, John E. Dominy, Yoon Jong Choi, Michael Jurczak, Nicola Tolliday, Joao Paulo Camporez, Helen Chim, Ji Hong Lim, Hai Bin Ruan, Xiaoyong Yang, Francisca Vazquez, Piotr Sicinski, Gerald I. Shulman, Pere Puigserver

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

Insulin constitutes a principal evolutionarily conserved hormonal axis for maintaining glucose homeostasis; dysregulation of this axis causes diabetes. PGC-1α (peroxisome-proliferator-Activated receptor-Î 3 coactivator-1α) links insulin signalling to the expression of glucose and lipid metabolic genes. The histone acetyltransferase GCN5 (general control non-repressed protein 5) acetylates PGC-1α and suppresses its transcriptional activity, whereas sirtuin 1 deacetylates and activates PGC-1α. Although insulin is a mitogenic signal in proliferative cells, whether components of the cell cycle machinery contribute to its metabolic action is poorly understood. Here we report that in mice insulin activates cyclin D1-cyclin-dependent kinase 4 (Cdk4), which, in turn, increases GCN5 acetyltransferase activity and suppresses hepatic glucose production independently of cell cycle progression. Through a cell-based high-throughput chemical screen, we identify a Cdk4 inhibitor that potently decreases PGC-1α acetylation. Insulin/GSK-3Î 2 (glycogen synthase kinase 3-beta) signalling induces cyclin D1 protein stability by sequestering cyclin D1 in the nucleus. In parallel, dietary amino acids increase hepatic cyclin D1 messenger RNA transcripts. Activated cyclin D1-Cdk4 kinase phosphorylates and activates GCN5, which then acetylates and inhibits PGC-1α activity on gluconeogenic genes. Loss of hepatic cyclin D1 results in increased gluconeogenesis and hyperglycaemia. In diabetic models, cyclin D1-Cdk4 is chronically elevated and refractory to fasting/feeding transitions; nevertheless further activation of this kinase normalizes glycaemia. Our findings show that insulin uses components of the cell cycle machinery in post-mitotic cells to control glucose homeostasis independently of cell division.

Original languageEnglish (US)
Pages (from-to)547-551
Number of pages5
JournalNature
Volume510
Issue number7506
DOIs
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • General

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