Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery

Amir Nasrolahi Shirazi; Karissa L. Paquin; Niall G. Howlett; Dindyal Mandal; Keykavous Parang

doi:10.3390/molecules190913319

Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery

Amir Nasrolahi Shirazi, Karissa L. Paquin, Niall G. Howlett, Dindyal Mandal, Keykavous Parang

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]₅ and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl₄ and the peptide generated peptide-capped gold nanoparticles. Here, [WR]₅ and [WR]₅-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]₅ and [WR]₅-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]₅ and [WR]₅-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.

Original language	English (US)
Pages (from-to)	13319-13331
Number of pages	13
Journal	Molecules
Volume	19
Issue number	9
DOIs	https://doi.org/10.3390/molecules190913319
State	Published - Aug 28 2014
Externally published	Yes

Keywords

Cyclic peptides
Gold nanoparticles
SiRNA delivery systems (DDS)
Small interfering RNA (siRNA) delivery

ASJC Scopus subject areas

Organic Chemistry
Medicine(all)

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10.3390/molecules190913319

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title = "Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery",

abstract = "Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.",

keywords = "Cyclic peptides, Gold nanoparticles, SiRNA delivery systems (DDS), Small interfering RNA (siRNA) delivery",

author = "Shirazi, {Amir Nasrolahi} and Paquin, {Karissa L.} and Howlett, {Niall G.} and Dindyal Mandal and Keykavous Parang",

year = "2014",

month = aug,

day = "28",

doi = "10.3390/molecules190913319",

language = "English (US)",

volume = "19",

pages = "13319--13331",

journal = "Molecules",

issn = "1420-3049",

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T1 - Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery

AU - Shirazi, Amir Nasrolahi

AU - Paquin, Karissa L.

AU - Howlett, Niall G.

AU - Mandal, Dindyal

AU - Parang, Keykavous

PY - 2014/8/28

Y1 - 2014/8/28

N2 - Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.

AB - Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.

KW - Cyclic peptides

KW - Gold nanoparticles

KW - SiRNA delivery systems (DDS)

KW - Small interfering RNA (siRNA) delivery

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U2 - 10.3390/molecules190913319

DO - 10.3390/molecules190913319

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SN - 1420-3049

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SP - 13319

EP - 13331

JO - Molecules

JF - Molecules

IS - 9

ER -

Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery

Abstract

Keywords

ASJC Scopus subject areas

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