Abstract
Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.
Original language | English (US) |
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Pages (from-to) | 13319-13331 |
Number of pages | 13 |
Journal | Molecules |
Volume | 19 |
Issue number | 9 |
DOIs | |
State | Published - Aug 28 2014 |
Externally published | Yes |
Keywords
- Cyclic peptides
- Gold nanoparticles
- SiRNA delivery systems (DDS)
- Small interfering RNA (siRNA) delivery
ASJC Scopus subject areas
- Organic Chemistry
- Medicine(all)