TY - JOUR
T1 - Cyclic-nucleotide-gated cation current and Ca2+-activated Cl current elicited by odorant in vertebrate olfactory receptor neurons
AU - Li, Rong Chang
AU - Ben-Chaim, Yair
AU - Yau, King Wai
AU - Lin, Chih Chun
N1 - Funding Information:
We thank Haiqing Zhao, Randall Reed, Jeremy Nathans (all of Johns Hopkins University School of Medicine), Samuel M. S. Wu (Baylor College of Medicine), and members of the K.-W.Y. laboratory for discussions and comments on the manuscript. We also thank the two reviewers for their extremely helpful comments. This work was supported by NIH Grant R01 DC14941 (to K.-W.Y.).
Publisher Copyright:
© 2016, National Academy of Sciences. All rights reserved.
PY - 2016/10/4
Y1 - 2016/10/4
N2 - Olfactory transduction in vertebrate olfactory receptor neurons (ORNs) involves primarily a cAMP-signaling cascade that leads to the opening of cyclic-nucleotide-gated (CNG), nonselective cation channels. The consequent Ca2+ influx triggers adaptation but also signal amplification, the latter by opening a Ca2+-activated Cl channel (ANO2) to elicit, unusually, an inward Cl current. Hence the olfactory response has inward CNG and Cl components that are in rapid succession and not easily separable. We report here success in quantitatively separating these two currents with respect to amplitude and time course over a broad range of odorant strengths. Importantly, we found that the Cl current is the predominant component throughout the olfactory dose-response relation, down to the threshold of signaling to the brain. This observation is very surprising given a recent report by others that the olfactory-signal amplification effected by the Ca2+-activated Cl current does not influence the behavioral olfactory threshold in mice.
AB - Olfactory transduction in vertebrate olfactory receptor neurons (ORNs) involves primarily a cAMP-signaling cascade that leads to the opening of cyclic-nucleotide-gated (CNG), nonselective cation channels. The consequent Ca2+ influx triggers adaptation but also signal amplification, the latter by opening a Ca2+-activated Cl channel (ANO2) to elicit, unusually, an inward Cl current. Hence the olfactory response has inward CNG and Cl components that are in rapid succession and not easily separable. We report here success in quantitatively separating these two currents with respect to amplitude and time course over a broad range of odorant strengths. Importantly, we found that the Cl current is the predominant component throughout the olfactory dose-response relation, down to the threshold of signaling to the brain. This observation is very surprising given a recent report by others that the olfactory-signal amplification effected by the Ca2+-activated Cl current does not influence the behavioral olfactory threshold in mice.
KW - Calcium-activated chloride channel
KW - Cyclic-nucleotide-gated channel
KW - Olfactory receptor neurons
KW - Olfactory transduction
KW - Signal amplification
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U2 - 10.1073/pnas.1613891113
DO - 10.1073/pnas.1613891113
M3 - Article
C2 - 27647918
AN - SCOPUS:84989827264
SN - 0027-8424
VL - 113
SP - 11078
EP - 11087
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 40
ER -