Cyclic caged morpholinos: Conformationally gated probes of embryonic gene function

Sayumi Yamazoe; Ilya A. Shestopalov; Elayne Provost; Steven D. Leach; James K. Chen

doi:10.1002/anie.201201690

Cyclic caged morpholinos: Conformationally gated probes of embryonic gene function

Sayumi Yamazoe, Ilya A. Shestopalov, Elayne Provost, Steven D. Leach, James K. Chen

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Feeling a bit cagey: Morpholino-based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.

Original language	English (US)
Pages (from-to)	6908-6911
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	28
DOIs	https://doi.org/10.1002/anie.201201690
State	Published - Jul 9 2012
Externally published	Yes

Keywords

antisense oligonucleotides
developmental biology
gene expression
oligonucleotides
zebrafish

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201201690

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title = "Cyclic caged morpholinos: Conformationally gated probes of embryonic gene function",

abstract = "Feeling a bit cagey: Morpholino-based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.",

keywords = "antisense oligonucleotides, developmental biology, gene expression, oligonucleotides, zebrafish",

author = "Sayumi Yamazoe and Shestopalov, {Ilya A.} and Elayne Provost and Leach, {Steven D.} and Chen, {James K.}",

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AU - Shestopalov, Ilya A.

AU - Provost, Elayne

AU - Leach, Steven D.

AU - Chen, James K.

PY - 2012/7/9

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KW - developmental biology

KW - gene expression

KW - oligonucleotides

KW - zebrafish

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Cyclic caged morpholinos: Conformationally gated probes of embryonic gene function

Abstract

Keywords

ASJC Scopus subject areas

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