TY - JOUR
T1 - Cutting edge
T2 - SWI/SNF mediates antisense Igh transcription and locus-wide accessibility in B cell precursors
AU - Osipovich, Oleg A.
AU - Subrahmanyam, Ramesh
AU - Pierce, Steven
AU - Sen, Ranjan
AU - Oltz, Eugene M.
PY - 2009/8/1
Y1 - 2009/8/1
N2 - The stepwise process of Ag receptor gene assembly, termed V(D)J recombination, is coordinated during lymphocyte development by sweeping changes in chromatin that permit or deny access to a single recombinase enzyme. We now show that switching/sucrose nonfermenting (SWI/SNF) chromatin remodeling complexes are recruited to the Igh locus by an enhancer-dependent process and that these complexes are essential for generating recombinase accessibility throughout the locus. Depletion of SWI/SNF in pro-B cells also inhibits antisense transcription through all clusters of Igh gene segments, a pioneering process that has been implicated in the initial opening of chromatin. We conclude that SWI/SNF complexes play multiple roles in Igh gene assembly, ranging from initial locus activation to the spreading and maintenance of chromatin accessibility over large VH, DH, and J H domains.
AB - The stepwise process of Ag receptor gene assembly, termed V(D)J recombination, is coordinated during lymphocyte development by sweeping changes in chromatin that permit or deny access to a single recombinase enzyme. We now show that switching/sucrose nonfermenting (SWI/SNF) chromatin remodeling complexes are recruited to the Igh locus by an enhancer-dependent process and that these complexes are essential for generating recombinase accessibility throughout the locus. Depletion of SWI/SNF in pro-B cells also inhibits antisense transcription through all clusters of Igh gene segments, a pioneering process that has been implicated in the initial opening of chromatin. We conclude that SWI/SNF complexes play multiple roles in Igh gene assembly, ranging from initial locus activation to the spreading and maintenance of chromatin accessibility over large VH, DH, and J H domains.
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U2 - 10.4049/jimmunol.0900896
DO - 10.4049/jimmunol.0900896
M3 - Article
C2 - 19596997
AN - SCOPUS:68149179271
SN - 0022-1767
VL - 183
SP - 1509
EP - 1513
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -