TY - JOUR
T1 - Current approaches for efficient genetic editing in human pluripotent stem cells
AU - Mukherjee-Clavin, Bipasha
AU - Tomishima, Mark
AU - Lee, Gabsang
N1 - Funding Information:
We would like to thank members of the Lee laboratory for valuable discussions on the manuscript. The Robertson Investigator Award from the New York Stem Cell Foundation (G.L.) and the Maryland Stem Cell Research Fund (G.L.) supported experiments in the Lee laboratory. The Starr Foundation and NYSTEM have supported experiments in the Tomishima lab.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2013/10
Y1 - 2013/10
N2 - Human pluripotent stem cells have been much anticipated as a powerful system to study developmental events, model genetic disorders, and serve as a source of autologous cells for cell therapy in genetic disorders. Precise genetic manipulation is crucial to all these applications, and many recent advances have been made in site specific nuclease systems like zinc finger nucleases, TALENs, and CRISPR/Cas. In this review, we address the importance of site-specific genome modification and how this technology can be applied to manipulate human pluripotent stem cells.
AB - Human pluripotent stem cells have been much anticipated as a powerful system to study developmental events, model genetic disorders, and serve as a source of autologous cells for cell therapy in genetic disorders. Precise genetic manipulation is crucial to all these applications, and many recent advances have been made in site specific nuclease systems like zinc finger nucleases, TALENs, and CRISPR/Cas. In this review, we address the importance of site-specific genome modification and how this technology can be applied to manipulate human pluripotent stem cells.
KW - CRISPR/Cas
KW - TALEN
KW - ZFN
KW - gene targeting
KW - human pluripotent stem cells
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U2 - 10.1007/s11515-013-1275-x
DO - 10.1007/s11515-013-1275-x
M3 - Review article
AN - SCOPUS:84884900622
SN - 1674-7984
VL - 8
SP - 461
EP - 467
JO - Frontiers in Biology
JF - Frontiers in Biology
IS - 5
ER -