TY - JOUR
T1 - Crystallization and X-ray diffraction studies of a 434 Cro-DNA complex
AU - Wolberger, Cynthia
AU - Harrison, Stephen C.
N1 - Funding Information:
We thank Mark Ptashne, in whose laboratory the isolation and initial crystallization of 434 Cro was carried out, and Robin Wharton, who helped to devise the purification and constructed overproducing plasmids. This work was supported by an K’IH grant (GM29109) to S.C.H. and Mark Ptashne.
PY - 1987/8/20
Y1 - 1987/8/20
N2 - Crystals have been obtained of the bacteriophage 434 Cro protein bound to a synthetic DNA operator. An analysis of the packing shows that the complexes stack end-to-end along crystallographic axis, forming long rods with non-crystallographic 113 screw symmetry. The average number of DNA base-pairs per turn is 10.27, which is somewhat more overwound as compared with the 434 repressor-DNA crystals of Anderson et al. Diffraction extends to 3 Å along the rod direction and to 5 Å in perpendicular directions.
AB - Crystals have been obtained of the bacteriophage 434 Cro protein bound to a synthetic DNA operator. An analysis of the packing shows that the complexes stack end-to-end along crystallographic axis, forming long rods with non-crystallographic 113 screw symmetry. The average number of DNA base-pairs per turn is 10.27, which is somewhat more overwound as compared with the 434 repressor-DNA crystals of Anderson et al. Diffraction extends to 3 Å along the rod direction and to 5 Å in perpendicular directions.
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U2 - 10.1016/0022-2836(87)90419-0
DO - 10.1016/0022-2836(87)90419-0
M3 - Article
C2 - 3681985
AN - SCOPUS:0023660878
SN - 0022-2836
VL - 196
SP - 951
EP - 954
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 4
ER -