TY - JOUR
T1 - Crystallization and preliminary X-ray diffraction analysis of the Saccharomyces cerevisiae Ran-binding protein Mog1p
AU - Baker, Rosanna P.
AU - Stewart, Murray
PY - 2000/2/1
Y1 - 2000/2/1
N2 - Mog1p binds the Ras-family GTPase Ran/Gsp1p, which has a central role in nucleocytoplasmic transport and cell-cycle progression. Overexpression of MOG1 is able to suppress temperature-sensitive gsp1 mutants in yeast; Δmog1 null mutants display temperature-sensitive defects in nuclear trafficking. Orthorhombic crystals of bacterially expressed Mog1p that diffract to beyond 2 Å resolution using synchrotron radiation have been obtained. The crystals have P212121 symmetry, with unit-cell parameters a = 39.67, b = 51.96, c = 112.23 Å, a Matthews coefficient of 2.44 Å3 Da-1, an estimated solvent content of 49.5% and one chain in the asymmetric unit.
AB - Mog1p binds the Ras-family GTPase Ran/Gsp1p, which has a central role in nucleocytoplasmic transport and cell-cycle progression. Overexpression of MOG1 is able to suppress temperature-sensitive gsp1 mutants in yeast; Δmog1 null mutants display temperature-sensitive defects in nuclear trafficking. Orthorhombic crystals of bacterially expressed Mog1p that diffract to beyond 2 Å resolution using synchrotron radiation have been obtained. The crystals have P212121 symmetry, with unit-cell parameters a = 39.67, b = 51.96, c = 112.23 Å, a Matthews coefficient of 2.44 Å3 Da-1, an estimated solvent content of 49.5% and one chain in the asymmetric unit.
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U2 - 10.1107/S0907444999016856
DO - 10.1107/S0907444999016856
M3 - Article
C2 - 10666614
AN - SCOPUS:0034141380
SN - 0907-4449
VL - 56
SP - 229
EP - 231
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 2
ER -