Crystallization and preliminary crystallographic analysis of the Escherichia coli water channel AqpZ

Brenda V. Daniels, Jian Sheng Jiang, Dax Fu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

AqpZ is a 24 kDa integral membrane protein that facilitates water movement across the plasma membrane of Escherichia coli. In this study, the first crystallization and preliminary X-ray analysis of AqpZ are described. AqpZ was overexpressed and purified with a yield of 13 mg of purified AqpZ per litre of cell culture. The purified AqpZ was shown to be a monodisperse species consisting of tetrameric protein-detergent complexes. A crystallization condition for producing diffraction-quality crystals was identified. Initial X-ray analysis indicated that the diffraction limit of AqpZ extended to 3.6 Å. Crystals were found to belong to space groups P4122 or P4322, with unit-cell parameters a = b = 119.04, c = 380.23 Å.

Original languageEnglish (US)
Pages (from-to)561-563
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume60
Issue number3
DOIs
StatePublished - Mar 2004
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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