CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Kyra L. Feuer; Marah H. Wahbeh; Christian Yovo; Eman Rabie; Anh Thu N. Lam; Sara Abdollahi; Lindsay J. Young; Bailey Rike; Akul Umamageswaran; Dimitrios Avramopoulos

doi:10.1038/s41598-022-16004-w

CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

Kyra L. Feuer, Marah H. Wahbeh, Christian Yovo, Eman Rabie, Anh Thu N. Lam, Sara Abdollahi, Lindsay J. Young, Bailey Rike, Akul Umamageswaran, Dimitrios Avramopoulos

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

Original language	English (US)
Article number	11928
Journal	Scientific reports
Volume	12
Issue number	1
DOIs	https://doi.org/10.1038/s41598-022-16004-w
State	Published - Dec 2022

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abstract = "Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.",

author = "Feuer, {Kyra L.} and Wahbeh, {Marah H.} and Christian Yovo and Eman Rabie and Lam, {Anh Thu N.} and Sara Abdollahi and Young, {Lindsay J.} and Bailey Rike and Akul Umamageswaran and Dimitrios Avramopoulos",

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AU - Feuer, Kyra L.

AU - Wahbeh, Marah H.

AU - Yovo, Christian

AU - Rabie, Eman

AU - Lam, Anh Thu N.

AU - Abdollahi, Sara

AU - Young, Lindsay J.

AU - Rike, Bailey

AU - Umamageswaran, Akul

AU - Avramopoulos, Dimitrios

PY - 2022/12

Y1 - 2022/12

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AB - Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~ 2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

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CRISPR Del/Rei: a simple, flexible, and efficient pipeline for scarless genome editing

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