CRISPR deactivation in mammalian cells using photocleavable guide RNAs

Roger S. Zou, Yang Liu, Taekjip Ha

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to deactivate CRISPR-Cas systems on demand would improve the safety and applicability of genome editing. Here, we detail a protocol using photocleavable guide RNAs (pcRNAs) to deactivate CRISPR-Cas9 inside cells. We verify that deactivation is both rapid and complete by checking for insertion-deletion (indel) mutations using Sanger sequencing. This protocol will be useful for researchers interested in using pcRNAs to improve genome editing specificity, characterize the timescales of genome editing, and study cellular DNA damage responses. For complete details on the use and execution of this protocol, please refer to Zou et al. (2021).

Original languageEnglish (US)
Article number100909
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - Dec 17 2021

Keywords

  • Biotechnology and bioengineering
  • CRISPR
  • Cell Biology
  • Cell-based Assays
  • Genomics
  • Molecular Biology
  • Molecular/Chemical Probes
  • Sequencing

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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