CRISPR-Cas9-based photoactivatable transcription system

Yuta Nihongaki; Shun Yamamoto; Fuun Kawano; Hideyuki Suzuki; Moritoshi Sato

doi:10.1016/j.chembiol.2014.12.011

CRISPR-Cas9-based photoactivatable transcription system

Yuta Nihongaki, Shun Yamamoto, Fuun Kawano, Hideyuki Suzuki, Moritoshi Sato

Research output: Contribution to journal › Article › peer-review

Abstract

Targeted endogenous gene activation is necessary for understanding complex gene networks and has great potential in medical and industrial applications. The CRISPR-Cas system offers simple and powerful tools for this purpose. However, these CRISPR-Cas-based tools for activating user-defined genes are unable to offer precise temporal control of gene expression, despite the fact that many biological phenomena are regulated by highly dynamic patterns of gene expression. Here we created a light-inducible, user-defined, endogenous gene activation system based on CRISPR-Cas9. We demonstrated that this CRISPR-Cas9-based transcription system can allow rapid and reversible targeted gene activation by light. In addition, using this system, we have exemplified photoactivation of multiple user-defined endogenous genes in mammalian cells. The present CRISPR-Cas9-based transcription system offers simple and versatile approaches for precise endogenous gene activation in basic biological research and biotechnology applications.

Original language	English (US)
Pages (from-to)	169-174
Number of pages	6
Journal	Chemistry and Biology
Volume	22
Issue number	2
DOIs	https://doi.org/10.1016/j.chembiol.2014.12.011
State	Published - Feb 19 2015
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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10.1016/j.chembiol.2014.12.011

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abstract = "Targeted endogenous gene activation is necessary for understanding complex gene networks and has great potential in medical and industrial applications. The CRISPR-Cas system offers simple and powerful tools for this purpose. However, these CRISPR-Cas-based tools for activating user-defined genes are unable to offer precise temporal control of gene expression, despite the fact that many biological phenomena are regulated by highly dynamic patterns of gene expression. Here we created a light-inducible, user-defined, endogenous gene activation system based on CRISPR-Cas9. We demonstrated that this CRISPR-Cas9-based transcription system can allow rapid and reversible targeted gene activation by light. In addition, using this system, we have exemplified photoactivation of multiple user-defined endogenous genes in mammalian cells. The present CRISPR-Cas9-based transcription system offers simple and versatile approaches for precise endogenous gene activation in basic biological research and biotechnology applications.",

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AU - Kawano, Fuun

AU - Suzuki, Hideyuki

AU - Sato, Moritoshi

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CRISPR-Cas9-based photoactivatable transcription system

Abstract

ASJC Scopus subject areas

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