Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching

Shuming Zhang, Xi Liu, Sebastian F. Barreto-Ortiz, Yixuan Yu, Brian P. Ginn, Nicholas A. DeSantis, Daphne L. Hutton, Warren L. Grayson, Fu Zhai Cui, Brian A. Korgel, Sharon Gerecht, Hai Quan Mao

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


Hydrogels have been widely used for 3-dimensional (3D) cell culture and tissue regeneration due to their tunable biochemical and physicochemical properties as well as their high water content, which resembles the aqueous microenvironment of the natural extracellular matrix. While many properties of natural hydrogel matrices are modifiable, their intrinsic isotropic structure limits the control over cellular organization, which is critical to restore tissue function. Here we report a generic approach to incorporate alignment topography inside the hydrogel matrix using a combination of electrical and mechanical stretching. Hydrogel fibres with uniaxial alignment were prepared from aqueous solutions of natural polymers such as alginate, fibrin, gelatin, and hyaluronic acid under ambient conditions. The unique internal alignment feature drastically enhances the mechanical properties of the hydrogel microfibres. Furthermore, the facile, organic solvent-free processing conditions are amenable to the incorporation of live cells within the hydrogel fibre or on the fibre surface; both approaches effectively induce cellular alignment. This work demonstrates a versatile and scalable strategy to create aligned hydrogel microfibres from various natural polymers.

Original languageEnglish (US)
Pages (from-to)3243-3251
Number of pages9
Issue number10
StatePublished - Mar 2014


  • Alginate
  • Fibrin
  • Hyaluronic acid
  • Hydrogel
  • Microstructure

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


Dive into the research topics of 'Creating polymer hydrogel microfibres with internal alignment via electrical and mechanical stretching'. Together they form a unique fingerprint.

Cite this