CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Meir Shamay; Nicholas Hand; M. Victor Lemas; Henry B. Koon; Susan E. Krown; John Wrangle; Prashant Desai; Juan Carlos Ramos; Richard F. Ambinder

doi:10.1093/infdis/jis032

CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Meir Shamay, Nicholas Hand, M. Victor Lemas, Henry B. Koon, Susan E. Krown, John Wrangle, Prashant Desai, Juan Carlos Ramos, Richard F. Ambinder

School of Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

Original language	English (US)
Pages (from-to)	1095-1099
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	205
Issue number	7
DOIs	https://doi.org/10.1093/infdis/jis032
State	Published - Apr 1 2012

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1093/infdis/jis032

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title = "CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA",

abstract = "We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.",

author = "Meir Shamay and Nicholas Hand and Lemas, {M. Victor} and Koon, {Henry B.} and Krown, {Susan E.} and John Wrangle and Prashant Desai and Ramos, {Juan Carlos} and Ambinder, {Richard F.}",

note = "Funding Information: Financial support. This work was supported by grants from the National Institutes of Health (P50CA96888, U01CA121947, P30CA006973, P01CA113239). Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.",

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doi = "10.1093/infdis/jis032",

language = "English (US)",

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AU - Shamay, Meir

AU - Hand, Nicholas

AU - Lemas, M. Victor

AU - Koon, Henry B.

AU - Krown, Susan E.

AU - Wrangle, John

AU - Desai, Prashant

AU - Ramos, Juan Carlos

AU - Ambinder, Richard F.

N1 - Funding Information: Financial support. This work was supported by grants from the National Institutes of Health (P50CA96888, U01CA121947, P30CA006973, P01CA113239). Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

PY - 2012/4/1

Y1 - 2012/4/1

N2 - We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

AB - We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

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ER -

CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Abstract

ASJC Scopus subject areas

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