CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

Angela H. Ting; Kam Wing Jair; Hiromu Suzuki; Ray Whay Chiu Yen; Stephen B. Baylin; Kornel E. Schuebel

doi:10.1038/ng1365

CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

Angela H. Ting, Kam Wing Jair, Hiromu Suzuki, Ray Whay Chiu Yen, Stephen B. Baylin, Kornel E. Schuebel

School of Medicine

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.

Original language	English (US)
Pages (from-to)	582-584
Number of pages	3
Journal	Nature genetics
Volume	36
Issue number	6
DOIs	https://doi.org/10.1038/ng1365
State	Published - Jun 2004

ASJC Scopus subject areas

Genetics

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title = "CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1",

abstract = "The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.",

author = "Ting, {Angela H.} and Jair, {Kam Wing} and Hiromu Suzuki and Yen, {Ray Whay Chiu} and Baylin, {Stephen B.} and Schuebel, {Kornel E.}",

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AU - Ting, Angela H.

AU - Jair, Kam Wing

AU - Suzuki, Hiromu

AU - Yen, Ray Whay Chiu

AU - Baylin, Stephen B.

AU - Schuebel, Kornel E.

PY - 2004/6

Y1 - 2004/6

N2 - The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.

AB - The role of the primary mammalian DNA methyltransferase, DNMT1, in maintaining CpG island methylation in human colon cancer cells has recently been questioned. This controversy has arisen from discrepancies between genetic knockout and RNA interference-mediated knockdown studies. Here, we re-examined the RNA interference-based approach and found that hypermethylation of single-copy genes is maintained in cells transiently and stably depleted of DNMT1.

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JO - Nature genetics

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CpG island hypermethylation is maintained in human colorectal cancer cells after RNAi-mediated depletion of DNMT1

Abstract

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