Coverage recommendations for methylation analysis by whole-genome bisulfite sequencing

Michael J. Ziller, Kasper D. Hansen, Alexander Meissner, Martin J. Aryee

Research output: Contribution to journalArticlepeer-review

133 Scopus citations


Whole-genome bisulfite sequencing (WGBS) allows genome-wide DNA methylation profiling, but the associated high sequencing costs continue to limit its widespread application. We used several high-coverage reference data sets to experimentally determine minimal sequencing requirements. We present data-derived recommendations for minimum sequencing depth for WGBS libraries, highlight what is gained with increasing coverage and discuss the trade-off between sequencing depth and number of assayed replicates.

Original languageEnglish (US)
Pages (from-to)230-232
Number of pages3
JournalNature Methods
Issue number3
StatePublished - Feb 26 2015

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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