Coupling antibody to cyanogen bromide-activated sepharose

Jennifer M. Kavran, Daniel J. Leahy

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations


Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification (see Immunoaffinity purification of proteins).

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Number of pages8
ISBN (Print)9780124201194
StatePublished - 2014

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Antibody and resin preparation
  • Antigen-antibody reactions
  • Ascitic fluid
  • Coupling antibody to resin
  • Cyanogen bromide (CNBr)-activated Sepharose
  • Hybridoma
  • Resin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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