Correlation of collagen organization with polarization sensitive imaging of in vitro cartilage: Implications for osteoarthritis

W. Drexler, D. Stamper, C. Jesser, X. Li, C. Pitris, K. Saunders, S. Martin, M. B. Lodge, J. G. Fujimoto, M. E. Brezinski

Research output: Contribution to journalArticlepeer-review

135 Scopus citations


Objective. Optical coherence tomography (OCT), a new method of high resolution imaging, has shown feasibility for assessing articular cartilage to identify early changes in osteoarthritis (OA) and monitor therapy. OCT is analogous to ultrasound, measuring the intensity of backreflected infrared light rather than sound. The resolution of this technology is up to 25 times higher than existing methods. We investigated the correlation between changes observed by OCT and the degree of collagen organization in OA cartilage. Methods. Polarization sensitive OCT (PSOCT) imaging was used to assess changes in cartilage collagen organization in vitro. Results. The presence (or absence) of PSOCT changes correlated with collagen organization (or disorganization) on histology as assessed by picrosirius polarization microscopy (no significant difference). In multiple cases, cartilage was abnormal by both PSOCT and polarization microscopy, but was grossly normal by routine staining, showing cartilage thickness > 2 mm and no fibrillations. Conclusion. This in vitro study suggests PSOCT changes in cartilage are due to the state of collagen organization. The combination of high resolution structural imaging and birefringence detection make OCT a potentially powerful technology for early assessment of OA.

Original languageEnglish (US)
Pages (from-to)1311-1318
Number of pages8
JournalJournal of Rheumatology
Issue number6
StatePublished - 2001
Externally publishedYes


  • Articular cartilage
  • Coherence
  • Collagen magnetic resonance imaging
  • Imaging
  • Optical
  • Osteoarthritis
  • Tomography

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Correlation of collagen organization with polarization sensitive imaging of in vitro cartilage: Implications for osteoarthritis'. Together they form a unique fingerprint.

Cite this