TY - JOUR
T1 - Correlating 3D morphology with molecular pathology
T2 - Fibrotic remodelling in human lung biopsies
AU - Kellner, Manuela
AU - Wehling, Judith
AU - Warnecke, Gregor
AU - Heidrich, Marko
AU - Izykowski, Nicole
AU - Vogel-Claussen, Jens
AU - Lorbeer, Raoul Amadeus
AU - Antonopoulos, Georgios
AU - Janciauskiene, Sabina
AU - Grothausmann, Roman
AU - Knudsen, Lars
AU - Ripken, Tammo
AU - Meyer, Heiko
AU - Kreipe, Hans
AU - Ochs, Matthias
AU - Jonigk, Danny
AU - Kühnel, Mark Philipp
N1 - Funding Information:
We would like to express our sincere gratitude to Susanne Kuhlmann and Regina Engelhardt for their excellent technical assistance, as well as Gareth Griffiths and Sheila Fryk for revising the text. Additionally, we would like to thank the developers and mailing list members of the open-source programmes Fiji (fiji.sc/Fiji), ITK-SNAP (itksnap.org/pmwiki/pmwiki.php), Paraview ( paraview.org), ITK (itk.org), VTK (vtk.org), VMTK (vmtk.org), ImageMagick (imagemagick.org) and mencoder (mplayerhq.hu).
Publisher Copyright:
© 2015, BMJ Publishing Group. All rights reserved.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - Assessing alterations of the parenchymal architecture is essential in understanding fibrosing interstitial lung diseases. Here, we present a novel method to visualise fibrotic remodelling in human lungs and correlate morphological three-dimensional (3D) data with gene and protein expression in the very same sample. The key to our approach is a novel embedding resin that clears samples to full optical transparency and simultaneously allows 3D laser tomography and preparation of sections for histology, immunohistochemistry and RNA isolation. Correlating 3D laser tomography with molecular diagnostic techniques enables new insights into lung diseases. This approach has great potential to become an essential tool in pulmonary research.
AB - Assessing alterations of the parenchymal architecture is essential in understanding fibrosing interstitial lung diseases. Here, we present a novel method to visualise fibrotic remodelling in human lungs and correlate morphological three-dimensional (3D) data with gene and protein expression in the very same sample. The key to our approach is a novel embedding resin that clears samples to full optical transparency and simultaneously allows 3D laser tomography and preparation of sections for histology, immunohistochemistry and RNA isolation. Correlating 3D laser tomography with molecular diagnostic techniques enables new insights into lung diseases. This approach has great potential to become an essential tool in pulmonary research.
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U2 - 10.1136/thoraxjnl-2015-207131
DO - 10.1136/thoraxjnl-2015-207131
M3 - Article
C2 - 26108569
AN - SCOPUS:84957847737
SN - 0040-6376
VL - 70
SP - 1197
EP - 1198
JO - Thorax
JF - Thorax
IS - 12
ER -