Conversion of Quiescent Niche Cells to Somatic Stem Cells Causes Ectopic Niche Formation in the Drosophila Testis

Phylis Hétié; Margaret de Cuevas; Erika Matunis

doi:10.1016/j.celrep.2014.03.058

Conversion of Quiescent Niche Cells to Somatic Stem Cells Causes Ectopic Niche Formation in the Drosophila Testis

Phylis Hétié, Margaret de Cuevas, Erika Matunis

School of Medicine

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Adult stem cells reside in specialized regulatory microenvironments, or niches, where local signals ensure stem cell maintenance. The Drosophila testis contains a well-characterized niche wherein signals from postmitotic hub cells promote maintenance of adjacent germline stem cells and somatic cyst stem cells (CySCs). Hub cells were considered to be terminally differentiated; here, we show that they can give rise to CySCs. Genetic ablation of CySCs triggers hub cells to transiently exit quiescence, delaminate from the hub, and convert into functional CySCs. Ectopic Cyclin D-Cdk4 expression in hub cells is also sufficient to trigger their conversion into CySCs. In both cases, this conversion causes the formation of multiple ectopic niches over time. Therefore, our work provides a model for understanding how oncogenic mutations in quiescent niche cells could promote loss of quiescence, changes in cell fate, and aberrant niche expansion.

Original language	English (US)
Pages (from-to)	715-721
Number of pages	7
Journal	Cell Reports
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/j.celrep.2014.03.058
State	Published - 2014

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Conversion of Quiescent Niche Cells to Somatic Stem Cells Causes Ectopic Niche Formation in the Drosophila Testis",

abstract = "Adult stem cells reside in specialized regulatory microenvironments, or niches, where local signals ensure stem cell maintenance. The Drosophila testis contains a well-characterized niche wherein signals from postmitotic hub cells promote maintenance of adjacent germline stem cells and somatic cyst stem cells (CySCs). Hub cells were considered to be terminally differentiated; here, we show that they can give rise to CySCs. Genetic ablation of CySCs triggers hub cells to transiently exit quiescence, delaminate from the hub, and convert into functional CySCs. Ectopic Cyclin D-Cdk4 expression in hub cells is also sufficient to trigger their conversion into CySCs. In both cases, this conversion causes the formation of multiple ectopic niches over time. Therefore, our work provides a model for understanding how oncogenic mutations in quiescent niche cells could promote loss of quiescence, changes in cell fate, and aberrant niche expansion.",

author = "Phylis H{\'e}ti{\'e} and {de Cuevas}, Margaret and Erika Matunis",

note = "Funding Information: We thank J. Abrams, S. DiNardo, D. Drummond-Barbosa, B. Edgar, D. McKearin, B. Ohlstein, A. Spradling, the Bloomington Drosophila Stock Center, and the Drosophila Genetic Resource Center for flies; E. Bach, C. Field, D. Godt, R. Lehmann, and the Developmental Studies Hybridoma Bank for antibodies; L. Greenspan, M. Matunis, R. Stine, and Q. Ma for comments; and M. Fuller and J. Lim for sharing data prior to publication. This work was supported by NIH grants RO1HD040307 and RO1HD052937 (to E.M.). ",

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N1 - Funding Information: We thank J. Abrams, S. DiNardo, D. Drummond-Barbosa, B. Edgar, D. McKearin, B. Ohlstein, A. Spradling, the Bloomington Drosophila Stock Center, and the Drosophila Genetic Resource Center for flies; E. Bach, C. Field, D. Godt, R. Lehmann, and the Developmental Studies Hybridoma Bank for antibodies; L. Greenspan, M. Matunis, R. Stine, and Q. Ma for comments; and M. Fuller and J. Lim for sharing data prior to publication. This work was supported by NIH grants RO1HD040307 and RO1HD052937 (to E.M.).

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N2 - Adult stem cells reside in specialized regulatory microenvironments, or niches, where local signals ensure stem cell maintenance. The Drosophila testis contains a well-characterized niche wherein signals from postmitotic hub cells promote maintenance of adjacent germline stem cells and somatic cyst stem cells (CySCs). Hub cells were considered to be terminally differentiated; here, we show that they can give rise to CySCs. Genetic ablation of CySCs triggers hub cells to transiently exit quiescence, delaminate from the hub, and convert into functional CySCs. Ectopic Cyclin D-Cdk4 expression in hub cells is also sufficient to trigger their conversion into CySCs. In both cases, this conversion causes the formation of multiple ectopic niches over time. Therefore, our work provides a model for understanding how oncogenic mutations in quiescent niche cells could promote loss of quiescence, changes in cell fate, and aberrant niche expansion.

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Conversion of Quiescent Niche Cells to Somatic Stem Cells Causes Ectopic Niche Formation in the Drosophila Testis

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