Controlling P element insertional mutagenesis

Lynn Cooley, Celeste Berg, Allan Spradling

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Two new methods for insertional mutagenesis in Drosophila are now available. They are based on the ability to control experimentally the number of stable P element insertions per mutagenized strain. One method produces about 10 insertions per genome and has the potential to replace hybrid dysgenesis as a means for cloning genes by transposon tagging. The other mobilizes elements less frequently, producing strains containing a single P element that can be correlated with any resulting mutant phenotype. A library of single-element strains is currently being assembled and will be a valuable resource for Drosophila molecular genetics.

Original languageEnglish (US)
Pages (from-to)254-258
Number of pages5
JournalTrends in Genetics
Issue number9
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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