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Continuous flow digital loop-mediated isothermal amplification (LAMP) in droplets

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Digital PCR is a newly emerging technology, very useful in precise nucleic acid quantification at single molecule level, even in the presence of large concentration of background DNA. However, the current commercially available instruments for digital PCR are limited in application due to a fragmented workflow requiring multiple instruments and low throughput. Here we propose a continuous flow digital loop-mediated isothermal amplification (LAMP) technique as an alternative to digital PCR. Successful application of this technique to digital nucleic acid detection demonstrates the potential of this technique to overcome all the issues associated with the digital PCR workflow.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1626-1628
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/TerritoryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Digital PCR
  • Droplets
  • Isothermal Amplification
  • LAMP

ASJC Scopus subject areas

  • Control and Systems Engineering

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