Construction of a Secondary Enclosure for UVB Irradiation of Mice

Justin Choi; Zachary A. Bordeaux; Gabriella Braun; Cole Davis; Varsha Parthasarathy; Junwen Deng; Mathew T. Taylor; Anusha Kambala; Hannah Cornman; Olusola Oladipo; Martin P. Alphonse; Cameron E. West; Shawn G. Kwatra; Madan M. Kwatra

doi:10.1016/j.xjidi.2022.100164

Construction of a Secondary Enclosure for UVB Irradiation of Mice

Justin Choi, Zachary A. Bordeaux, Gabriella Braun, Cole Davis, Varsha Parthasarathy, Junwen Deng, Mathew T. Taylor, Anusha Kambala, Hannah Cornman, Olusola Oladipo, Martin P. Alphonse, Cameron E. West, Shawn G. Kwatra, Madan M. Kwatra

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

UV irradiation is commonly used in murine models of skin cancers. Despite the popularity of using UVB rays to model photocarcinogenesis in animals, there is a lack of standardization in the secondary enclosures used to administer radiation. An appraisal of the literature also shows a general lack of details regarding the materials and procedures utilized in the fabrication of such enclosures. We present in this study a detailed overview of the construction of a UVB exposure chamber that successfully induces lesions in hairless mice. A standardized protocol for producing a UVB enclosure may reduce methodological variation in future studies seeking to investigate photocarcinogenesis in animals.

Original language	English (US)
Article number	100164
Journal	JID Innovations
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xjidi.2022.100164
State	Published - Jan 2023

ASJC Scopus subject areas

Dermatology

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10.1016/j.xjidi.2022.100164

Cite this

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title = "Construction of a Secondary Enclosure for UVB Irradiation of Mice",

abstract = "UV irradiation is commonly used in murine models of skin cancers. Despite the popularity of using UVB rays to model photocarcinogenesis in animals, there is a lack of standardization in the secondary enclosures used to administer radiation. An appraisal of the literature also shows a general lack of details regarding the materials and procedures utilized in the fabrication of such enclosures. We present in this study a detailed overview of the construction of a UVB exposure chamber that successfully induces lesions in hairless mice. A standardized protocol for producing a UVB enclosure may reduce methodological variation in future studies seeking to investigate photocarcinogenesis in animals.",

author = "Justin Choi and Bordeaux, {Zachary A.} and Gabriella Braun and Cole Davis and Varsha Parthasarathy and Junwen Deng and Taylor, {Mathew T.} and Anusha Kambala and Hannah Cornman and Olusola Oladipo and Alphonse, {Martin P.} and West, {Cameron E.} and Kwatra, {Shawn G.} and Kwatra, {Madan M.}",

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year = "2023",

month = jan,

doi = "10.1016/j.xjidi.2022.100164",

language = "English (US)",

volume = "3",

journal = "JID Innovations",

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AU - Choi, Justin

AU - Bordeaux, Zachary A.

AU - Braun, Gabriella

AU - Davis, Cole

AU - Parthasarathy, Varsha

AU - Deng, Junwen

AU - Taylor, Mathew T.

AU - Kambala, Anusha

AU - Cornman, Hannah

AU - Oladipo, Olusola

AU - Alphonse, Martin P.

AU - West, Cameron E.

AU - Kwatra, Shawn G.

AU - Kwatra, Madan M.

PY - 2023/1

Y1 - 2023/1

N2 - UV irradiation is commonly used in murine models of skin cancers. Despite the popularity of using UVB rays to model photocarcinogenesis in animals, there is a lack of standardization in the secondary enclosures used to administer radiation. An appraisal of the literature also shows a general lack of details regarding the materials and procedures utilized in the fabrication of such enclosures. We present in this study a detailed overview of the construction of a UVB exposure chamber that successfully induces lesions in hairless mice. A standardized protocol for producing a UVB enclosure may reduce methodological variation in future studies seeking to investigate photocarcinogenesis in animals.

AB - UV irradiation is commonly used in murine models of skin cancers. Despite the popularity of using UVB rays to model photocarcinogenesis in animals, there is a lack of standardization in the secondary enclosures used to administer radiation. An appraisal of the literature also shows a general lack of details regarding the materials and procedures utilized in the fabrication of such enclosures. We present in this study a detailed overview of the construction of a UVB exposure chamber that successfully induces lesions in hairless mice. A standardized protocol for producing a UVB enclosure may reduce methodological variation in future studies seeking to investigate photocarcinogenesis in animals.

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Construction of a Secondary Enclosure for UVB Irradiation of Mice

Abstract

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