Abstract
A new bacterial cloning vector, pGreenLD, derived from the triple substitution mutated Aequorea victoria green fluorescent protein (GFP-S65A, V68L, S72A), when expressed in E. coli produced colonies which showed yellow-green colour under daylight and strong green fluorescence under long-wave ultraviolet light. It can be a useful vector for selecting foreign DNA fragment which was inserted into multiple cloning site based on the loss of the yellow-green color/green fluorescence of E. coli cells attributable to the insertional inactivation of GFP production.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 487-489 |
| Number of pages | 3 |
| Journal | Acta Botanica Sinica |
| Volume | 41 |
| Issue number | 5 |
| State | Published - May 1 1999 |
| Externally published | Yes |
Keywords
- Cloning vector
- GFPmut2
- Green fluorescent protein
- pBluescript SK(+)
ASJC Scopus subject areas
- Biochemistry
- General Biochemistry, Genetics and Molecular Biology
- Plant Science
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