Connexin43 mRNA contains a functional internal ribosome entry site

Adam Schiavi; Alice Hudder; Rudolf Werner

doi:10.1016/S0014-5793(99)01699-3

Connexin43 mRNA contains a functional internal ribosome entry site

Adam Schiavi, Alice Hudder, Rudolf Werner

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

A reporter gene construct was used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%. A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES). Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR.

Original language	English (US)
Pages (from-to)	118-122
Number of pages	5
Journal	FEBS Letters
Volume	464
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01699-3
State	Published - Dec 31 1999
Externally published	Yes

Keywords

Connexin43
Internal ribosome entry site
Translation control
mRNA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)01699-3

Cite this

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title = "Connexin43 mRNA contains a functional internal ribosome entry site",

abstract = "A reporter gene construct was used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%. A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES). Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR.",

keywords = "Connexin43, Internal ribosome entry site, Translation control, mRNA",

author = "Adam Schiavi and Alice Hudder and Rudolf Werner",

note = "Funding Information: This study was supported by a Grant from the National Institute of Health (HD34152). DNA synthesis and sequence analysis was subsidized by the Sylvester Comprehensive Cancer Center through their DNA Core Facility.",

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doi = "10.1016/S0014-5793(99)01699-3",

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T1 - Connexin43 mRNA contains a functional internal ribosome entry site

AU - Schiavi, Adam

AU - Hudder, Alice

AU - Werner, Rudolf

N1 - Funding Information: This study was supported by a Grant from the National Institute of Health (HD34152). DNA synthesis and sequence analysis was subsidized by the Sylvester Comprehensive Cancer Center through their DNA Core Facility.

PY - 1999/12/31

Y1 - 1999/12/31

N2 - A reporter gene construct was used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%. A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES). Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR.

AB - A reporter gene construct was used to study the regulation of connexin43 (Cx43) expression, the major gap junction protein found in heart and uterus, in transfected cell lines. The construct had the firefly luciferase gene under the control of the Cx43 promoter. Inclusion of the 5'-untranslated region (UTR) of the mRNA in the construct increased luciferase expression by 70%. A bicistronic vector assay demonstrated that the Cx43 5'-UTR contains a strong internal ribosome entry site (IRES). Deletion analysis localized the IRES element to the upstream portion of the 5'-UTR.

KW - Connexin43

KW - Internal ribosome entry site

KW - Translation control

KW - mRNA

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Connexin43 mRNA contains a functional internal ribosome entry site

Abstract

Keywords

ASJC Scopus subject areas

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