Comprehensive profiling of antibody responses to the human anellome using programmable phage display

Thiagarajan Venkataraman, Harish Swaminathan, Cesar A. Arze, Sarah M. Jacobo, Agamoni Bhattacharyya, Tyler David, Dhananjay M. Nawandar, Simon Delagrave, Vinidhra Mani, Nathan L. Yozwiak, H. Benjamin Larman

Research output: Contribution to journalArticlepeer-review


Anelloviruses represent a major constituent of the commensal human virome; however, little is known about their immunobiology. Here, we present “AnelloScan,” a T7 phage library representing the open reading frame 1 (ORF1), ORF2, ORF3, and torque teno virus (TTV)-derived apoptosis-inducing protein (TAIP) sequences of more than 800 human anelloviruses and profile the antibody reactivities of serum samples from a cross-sectional cohort of 156 subjects by using phage-immunoprecipitation sequencing (PhIP-Seq). A majority of anellovirus peptides are not reactive in any of the subjects tested (n = ∼28,000; ∼85% of the library). Antibody-reactive peptides are largely restricted to the C-terminal region of the capsid protein ORF1. Moreover, using a longitudinal cohort of matched blood-transfusion donors and recipients, we find that most transmitted anelloviruses do not elicit a detectable antibody reactivity in the recipient and that the remainder elicit delayed responses appearing ∼100–150 days after transfusion.

Original languageEnglish (US)
Article number111754
JournalCell Reports
Issue number12
StatePublished - Dec 20 2022


  • AnelloScan
  • CP: Microbiology
  • PhIP-seq
  • TTV
  • VirScan
  • anelloviridae
  • anellovirus
  • antibodies
  • torque teno virus
  • transfusion

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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