TY - GEN
T1 - Compositional control of pDNA/lPEI nanoparticles using flash nanocomplexation to improve in vivo transfection efficiency and biocompatibility
AU - Hu, Yizong
AU - Liu, Heng Wen
AU - Minn, Il
AU - Pomper, Martin
AU - Mao, Hai Quan
N1 - Funding Information:
Declare of interest: This study is partially supported by a research contract from Cancer Targeting Systems, Inc. (CTS). The authors thank Chris Ullman and Christine Carrington from CTS for helpful discussions.
Publisher Copyright:
© 2019 Omnipress - All rights reserved.
PY - 2019
Y1 - 2019
N2 - Linear PEI (lPEI) has proven track record as an effective carrier for the delivery of therapeutic plasmid DNA (pDNA) for disease treatment. However, the lack of a reproducible method to produce pDNA/lPEI nanoparticles with controlled physicochemical characteristics and compositions has long hindered the development of these nanoparticles as a pharmaceutical product. We have employed a turbulent mixing methodology at high Reynolds numbers using the flash nanocomplexation (FNC) technique to achieve efficient and homogenous complexation of pDNA and lPEI. Through precise control of the kinetic conditions for the nanoparticle assembly, uniform nanoparticles with a well-defined composition was developed that presented benefit over both in vivo transfection efficiency and biocompatibility.
AB - Linear PEI (lPEI) has proven track record as an effective carrier for the delivery of therapeutic plasmid DNA (pDNA) for disease treatment. However, the lack of a reproducible method to produce pDNA/lPEI nanoparticles with controlled physicochemical characteristics and compositions has long hindered the development of these nanoparticles as a pharmaceutical product. We have employed a turbulent mixing methodology at high Reynolds numbers using the flash nanocomplexation (FNC) technique to achieve efficient and homogenous complexation of pDNA and lPEI. Through precise control of the kinetic conditions for the nanoparticle assembly, uniform nanoparticles with a well-defined composition was developed that presented benefit over both in vivo transfection efficiency and biocompatibility.
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M3 - Conference contribution
AN - SCOPUS:85065420002
T3 - Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
SP - 383
BT - Society for Biomaterials Annual Meeting and Exposition 2019
PB - Society for Biomaterials
T2 - 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
Y2 - 3 April 2019 through 6 April 2019
ER -