Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy

Peter C.M. Van Zul, Chrit T.W. Moonen, Patrick Faustino, James Pekar, Ofer Kaplan, Jack S. Cohen

Research output: Contribution to journalArticlepeer-review

179 Scopus citations

Abstract

A method is outlined that completely separates intracellular and extracellular information in NMR spectra of perfused cells. The technique uses diffusion weighting to exploit differences in motional properties between intra- and extracellular constituents. This allows monitoring of intracellular metabolism, and of transport of small drugs and nutrients through the cell membrane, under controlled physiological conditions. As a first example, proton spectra of drug-resistant MCF-7 human breast cancer cells are studied, and uptake of phenylalanine is monitored.

Original languageEnglish (US)
Pages (from-to)3228-3232
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number8
StatePublished - 1991
Externally publishedYes

Keywords

  • Breast cancer cells
  • Intracellular metabolism
  • Membrane permeability
  • Proton NMR
  • Transport

ASJC Scopus subject areas

  • General

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