TY - JOUR
T1 - Complement factor H and high-temperature requirement A-1 genotypes and treatment response of age-related macular degeneration
AU - Tsuchihashi, Takashi
AU - Mori, Keisuke
AU - Horie-Inoue, Kuniko
AU - Gehlbach, Peter L.
AU - Kabasawa, Sho
AU - Takita, Hiroyasu
AU - Ueyama, Kazuhiro
AU - Okazaki, Yasushi
AU - Inoue, Satoshi
AU - Awata, Takuya
AU - Katayama, Shigehiro
AU - Yoneya, Shin
N1 - Funding Information:
Supported in part by a Grant from the Eye Research Foundation for the Aged (KM), an Institutional Grant from the Medical Research Center, Saitama Medical University ( #20-1-2-02 , KM) and a grant-in-aid for scientific research ( 21592242 ) from the Ministry of Education, Culture and Science in Japan (KM).
PY - 2011/1
Y1 - 2011/1
N2 - Purpose: To determine whether there is an association between complement factor H (CFH), high-temperature requirement A-1 (HTRA1), vascular endothelial growth factor (VEGF), and pigment epithelium-derived factor (PEDF) genotypes and response to treatment with photodynamic therapy (PDT) for age-related macular degeneration (AMD) in a Japanese population. Design: Prospective, case-control study. Participants: One hundred ten patients with exudative AMD treated by verteporfin PDT were recruited prospectively at the Department of Ophthalmology, Saitama Medical University Hospital, Saitama, Japan. Methods: The patients were genotyped for 4 single nucleotide polymorphisms (SNPs; rs800292, rs1061170, rs1410996, rs2274700) in the CFH gene, a rs11200638-SNP in the HTRA1 gene, 3 SNPs (rs699947, rs1570360, rs2010963) in the VEGF gene, and 4 SNPs (rs12150053, rs12948385, rs9913583, rs1136287) in the PEDF gene using a TaqMan assay. Main Outcome Measures: The treatment outcomes and genotypes of CFH, HTRA1, VEGF, and PEDF polymorphisms. Results: Best-corrected visual acuity 1 year after PDT was significantly increased in patients with the HTRA1-rs11200638 GG genotype as compared with patients with the GA or AA genotypes (P = 2.9×10 -2, 7.0×10-4, respectively). The rate of recurrence in the 12-month period after PDT was also associated with HTRA1-rs11200638 genotype (P = 3.12×10-2). Patients with the AA genotype of HTRA1-rs11200638 had an approximately 6-fold greater risk of the recurrence than patients with the GG genotype (P = 5.58×10-3). Significant differences were demonstrated in the mean time interval from the initial treatment to the time of recurrence for the genotypes of CFH-rs1410996/rs2274700 (P = 8.50×10-3). Conclusions: The HTRA1-rs11200638 and CFH-rs1410996/-rs2274700 variants were associated with response to PDT in this study population. These variants may be used for genetic biomarkers to estimate visual outcomes and recurrences in the response to PDT with significant predictive power. Financial Disclosure(s): The authors have no proprietary or commercial interest in any of the materials discussed in this article.
AB - Purpose: To determine whether there is an association between complement factor H (CFH), high-temperature requirement A-1 (HTRA1), vascular endothelial growth factor (VEGF), and pigment epithelium-derived factor (PEDF) genotypes and response to treatment with photodynamic therapy (PDT) for age-related macular degeneration (AMD) in a Japanese population. Design: Prospective, case-control study. Participants: One hundred ten patients with exudative AMD treated by verteporfin PDT were recruited prospectively at the Department of Ophthalmology, Saitama Medical University Hospital, Saitama, Japan. Methods: The patients were genotyped for 4 single nucleotide polymorphisms (SNPs; rs800292, rs1061170, rs1410996, rs2274700) in the CFH gene, a rs11200638-SNP in the HTRA1 gene, 3 SNPs (rs699947, rs1570360, rs2010963) in the VEGF gene, and 4 SNPs (rs12150053, rs12948385, rs9913583, rs1136287) in the PEDF gene using a TaqMan assay. Main Outcome Measures: The treatment outcomes and genotypes of CFH, HTRA1, VEGF, and PEDF polymorphisms. Results: Best-corrected visual acuity 1 year after PDT was significantly increased in patients with the HTRA1-rs11200638 GG genotype as compared with patients with the GA or AA genotypes (P = 2.9×10 -2, 7.0×10-4, respectively). The rate of recurrence in the 12-month period after PDT was also associated with HTRA1-rs11200638 genotype (P = 3.12×10-2). Patients with the AA genotype of HTRA1-rs11200638 had an approximately 6-fold greater risk of the recurrence than patients with the GG genotype (P = 5.58×10-3). Significant differences were demonstrated in the mean time interval from the initial treatment to the time of recurrence for the genotypes of CFH-rs1410996/rs2274700 (P = 8.50×10-3). Conclusions: The HTRA1-rs11200638 and CFH-rs1410996/-rs2274700 variants were associated with response to PDT in this study population. These variants may be used for genetic biomarkers to estimate visual outcomes and recurrences in the response to PDT with significant predictive power. Financial Disclosure(s): The authors have no proprietary or commercial interest in any of the materials discussed in this article.
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U2 - 10.1016/j.ophtha.2010.04.007
DO - 10.1016/j.ophtha.2010.04.007
M3 - Article
C2 - 20678803
AN - SCOPUS:78650840561
SN - 0161-6420
VL - 118
SP - 93
EP - 100
JO - Ophthalmology
JF - Ophthalmology
IS - 1
ER -