Compatibility of Gd-DTPA perfusion and histologic studies of the brain

Richard G. Spencer, Kenneth W. Fishbein, Aiwu Cheng, Mark P. Mattson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Histology, including immunohistochemistry, and magnetic resonance imaging microscopy (μMRI) are complementary techniques for the analysis of brain structure. Therefore, μMRI analysis, often of formalin-fixed tissue, precedes histologic evaluation of the same experimental animal in many studies. However, the application of gadopentetate dimeglumine (Gd-DTPA), while of value for MRI studies, has an unknown effect on subsequent histology. We demonstrate here that for the mouse brain, histology with Nissl staining and immunostaining for microtubule-associated protein 2, using standard techniques for tissue preparation, are unaffected by prior perfusion of the tissue with Gd-DTPA. This conclusion was based on qualitative morphologic comparisons of stained sections, as well as quantification of mean immunofluorescence pixel intensities from Gd-treated (mean±S.D.=131.2±28.4; n=3) as compared to nontreated specimens (116.2±34.7; n=3, P=.7). Therefore, Gd-DTPA may be applied as a μMRI contrast agent in formalin-fixed brain tissue prior to histologic studies.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalMagnetic Resonance Imaging
Issue number1
StatePublished - Jan 2006
Externally publishedYes


  • Brain imaging
  • Gd-DTPA
  • Histology
  • Immunohistochemistry
  • MR microscopy

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics


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