Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

B. D. Kirkpatrick; Matthew D. Bentley; Anette M. Thern; Catherine J. Larsson; Cassandra Ventrone; Meera V. Sreenivasan; Lou Bourgeois

doi:10.1128/CDLI.12.9.1127-1129.2005

Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

B. D. Kirkpatrick, Matthew D. Bentley, Anette M. Thern, Catherine J. Larsson, Cassandra Ventrone, Meera V. Sreenivasan, Lou Bourgeois

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at ≤42 spots/10⁶ peripheral blood lymphocytes.

Original language	English (US)
Pages (from-to)	1127-1129
Number of pages	3
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	12
Issue number	9
DOIs	https://doi.org/10.1128/CDLI.12.9.1127-1129.2005
State	Published - Sep 2005
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

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10.1128/CDLI.12.9.1127-1129.2005

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Kirkpatrick, B. D., Bentley, M. D., Thern, A. M., Larsson, C. J., Ventrone, C., Sreenivasan, M. V., & Bourgeois, L. (2005). Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09). Clinical and Diagnostic Laboratory Immunology, 12(9), 1127-1129. https://doi.org/10.1128/CDLI.12.9.1127-1129.2005

Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09). / Kirkpatrick, B. D.; Bentley, Matthew D.; Thern, Anette M. et al.
In: Clinical and Diagnostic Laboratory Immunology, Vol. 12, No. 9, 09.2005, p. 1127-1129.

Research output: Contribution to journal › Article › peer-review

Kirkpatrick, BD, Bentley, MD, Thern, AM, Larsson, CJ, Ventrone, C, Sreenivasan, MV & Bourgeois, L 2005, 'Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)', Clinical and Diagnostic Laboratory Immunology, vol. 12, no. 9, pp. 1127-1129. https://doi.org/10.1128/CDLI.12.9.1127-1129.2005

Kirkpatrick BD, Bentley MD, Thern AM, Larsson CJ, Ventrone C, Sreenivasan MV et al. Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09). Clinical and Diagnostic Laboratory Immunology. 2005 Sep;12(9):1127-1129. doi: 10.1128/CDLI.12.9.1127-1129.2005

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Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

Abstract

ASJC Scopus subject areas

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